Comparable responses of osteoblast lineage cells to microstructured hydrophilic titanium-zirconium and microstructured hydrophilic titanium
Comparable responses of osteoblast lineage cells to microstructured hydrophilic titanium-zirconium and microstructured hydrophilic titanium
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DOI：
10.1111/clr.12855
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发表时间：
2017-07-01
影响因子：
4.3
通讯作者：
Boyan, Barbara D.
中科院分区：
文献类型：
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作者：
Lotz, Ethan M.;Olivares-Navarrete, Rene;Boyan, Barbara D.
虽然钛（Ti）通常用于牙科植入物，但钛合金材料正在开发以改善其物理材料性能。研究表明，人间充质干细胞（MSC）和正常人成骨细胞（NHOsts）的成骨细胞分化和成熟对微结构钛和钛铝钒（Ti6 Al 4V）表面的反应方式相似。本研究的目的是确定在微结构化TiZr表面上生长的成骨细胞谱系细胞是否是这种情况，以及它们的反应是否受到表面纳米纹理和亲水性的影响。（13- 17%Zr）盘通过大粒度喷砂和酸蚀刻进行改性，并在盐水溶液中储存，从而产生对应于市售植入物SLActive和Roxolid SLActive（InstitutStraumannAG，巴塞尔，瑞士）的复杂的微结构化和亲水性表面。随后的Ti modSLA和TiZr modSLA表面的特点和成骨标记物measured.ResultsEvaluation的物理参数显示，该制造方法是能够诱导的Ti和TiZr磁盘上的微结构和亲水性表面。总体而言，表面是相似的，但检测到纳米结构形态/密度和表面化学的差异。在Ti modSLA和TiZr modSLA，成骨细胞分化和成熟的标志物均增强，而炎性标志物减少与TCPS.ConclusionsThese结果表明，类似的阳性细胞反应的MSC和NHOsts培养时，Ti modSLA和TiZr modSLA。两种表面都是亲水性的，表明这种性质对成骨细胞系细胞的重要性。
ObjectivesAlthough titanium (Ti) is commonly used for dental implants, Ti alloy materials are being developed to improve their physical material properties. Studies indicate that osteoblast differentiation and maturation of human mesenchymal stem cells (MSCs) and normal human osteoblasts (NHOsts) respond to microstructured Ti and titanium-aluminum-vanadium (Ti6Al4V) surfaces in a similar manner. The goal of this study was to determine whether this is the case for osteoblast lineage cells grown on microstructured TiZr surfaces and whether their response is affected by surface nanotexture and hydrophilicity.Materials and MethodsGrade 4 Ti and TiZr (13-17% Zr) disks were modified by large grit sand-blasting and acid-etching with storage in saline solution, resulting in a complex microstructured and hydrophilic surface corresponding to the commercially available implants SLActive((R)) and Roxolid((R)) SLActive((R)) (Institut Straumann AG, Basel, Switzerland). The subsequent Ti modSLA and TiZr modSLA surfaces were characterized and osteogenic markers were measured.ResultsEvaluation of physical parameters revealed that the fabrication method was capable of inducing a microstructured and hydrophilic surface on both the Ti and TiZr disks. Overall, the surfaces were similar, but differences in nanostructure morphology/density and surface chemistry were detected. On Ti modSLA and TiZr modSLA, osteoblastic differentiation and maturation markers were enhanced in both MSCs and NHOsts, while inflammatory markers decreased compared with TCPS.ConclusionsThese results indicate a similar positive cell response of MSCs and NHOsts when cultured on Ti modSLA and TiZr modSLA. Both surfaces were hydrophilic, indicating the importance of this property to osteoblast lineage cells.