Identification of Nitrogen-Fixing Paenibacillus from Different Plant Rhizospheres and a Novel nifH Gene Detected in the P. stellifer
Identification of Nitrogen-Fixing Paenibacillus from Different Plant Rhizospheres and a Novel nifH Gene Detected in the P. stellifer
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DOI：
10.1134/s0026261711010097
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发表时间：
2011-02-01
期刊：
影响因子：
1.5
通讯作者：
Chen, S. F.
中科院分区：
文献类型：
--
作者：
Jin, H. J.;Tu, R.;Chen, S. F.
根据其在无氮培养基上的生长和对80 ℃ 15 min的抗性，从不同植物根际共获得534个菌株。在534个菌株中，有23个菌株具有nifH基因并表现出固氮酶活性。根据16 S rDNA序列测定、G + C含量测定和DNA-DNA杂交结果，将23个菌株分为4个单系群，均属于类芽孢杆菌属。nifH基因推导的氨基酸序列比对结果表明，聚类I中15个菌株与类芽孢杆菌属（Paenibacillus）的NifH同源性最高，聚类II中4个未培养的菌株与类芽孢杆菌属（Paenibacillus）的NifH同源性最高。研究表明，固氮类芽孢杆菌在不同生态系统中均有分布，并在不同植物根际中普遍存在。这是首次证实了黄绵马和星叶黄绵马具有固氮作用。在鉴定为星状假单胞菌的8个分离株中，在类芽孢杆菌中检测到一种新的nifH基因。
A total of 534 isolates were selectively obtained from different plant rhizospheres based on their growth on nitrogen-free medium and their resistance to 80 degrees C for 15 min. Of the 534 isolates, 23 isolates had nifH gene and exhibited nitrogenase activities. Based on 16S rDNA sequence, G + C content assay and DNA-DNA hybridization, the 23 isolates which divided into four monophyletic clusters were all belonged to the Paenibacillus genus. nifH gene deduced amino acid alignment aLnalysis revealed that cluster I, including 15 isolates, showed the highest NifH identity with Paenibacillus genus; while cluster II identified as P. stellifer by DNA-DNA hybridization was consistent with four uncultured bacterial clones. This study suggested that the nitrogen-fixing Paenibacillus were distributed in various ecosystems and prevalent in different plant rhizospheres. It was the first demonstration that nitrogen fixation existed in P. jamilae and P. stellifer. In eight isolates identified as P. stellifer species, a novel nifH gene was detected in Paenibacillus.