Characterization of the cytolytic T-lymphocyte response to a candidate vaccine strain of equine herpesvirus 1 in CBA mice.
Characterization of the cytolytic T-lymphocyte response to a candidate vaccine strain of equine herpesvirus 1 in CBA mice.
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CBA 小鼠中对马疱疹病毒 1 候选疫苗株的溶细胞 T 淋巴细胞反应的表征。
DOI：
10.1128/jvi.72.7.5366-5372.1998
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发表时间：
1998
影响因子：
5.4
通讯作者：
O'Callaghan,DJ
中科院分区：
文献类型：
--
作者：
Smith,PM;Zhang,Y;Jennings,SR;O'Callaghan,DJ
研究了CBA （H-2k）小鼠呼吸道感染马疱疹病毒1型（EHV-1）后的细胞溶解t淋巴细胞（CTL）反应。在小鼠鼻内接种减毒的EHV-1 KyA菌株，在感染后5天，在纵隔引流淋巴结中产生了初级病毒特异性CTL反应。ehv -1特异性CTL可以在感染消退后的26周内从脾脏重新激活，这表明产生了一个长寿命的记忆CTL群体。在实验前用抗体和补体处理CD8+T细胞，消除了原代和记忆群体中的CTL活性，表明该模型中的细胞溶解活性是由I类主要组织相容性复合物限制的CD8+T细胞介导的。单次接种KyA诱导对致病性EHV-1菌株RacL11感染的保护性免疫。将kya免疫供者的脾细胞过继转移到亚致死照射的受体中，导致肺中RacL11减少250倍以上。从转移的细胞中同时清除CD4+和CD8+T细胞会消除RacL11的清除，而单独选择性地清除任何一个亚群都没有什么影响。这些结果表明，两个淋巴细胞亚群都有助于病毒清除，任何一个亚群单独都是足够的。
The cytolytic T-lymphocyte (CTL) response to respiratory infection with equine herpesvirus 1 (EHV-1) in CBA (H-2k) mice was investigated. Intranasal (i.n.) inoculation of mice with the attenuated EHV-1 strain KyA resulted in the generation of a primary virus-specific CTL response in the draining mediastinal lymph nodes 5 days following infection. EHV-1-specific CTL could be restimulated from the spleen up to 26 weeks after the resolution of infection, indicating that a long-lived memory CTL population was generated. Depletion of CD8+T cells by treatment with antibody and complement prior to assay eliminated CTL activity from both primary and memory populations, indicating that cytolytic activity in this model was mediated by class I major histocompatibility complex-restricted, CD8+T cells. A single i.n. inoculation with KyA induced protective immunity against infection with the pathogenic EHV-1 strain, RacL11. The adoptive transfer of splenocytes from KyA-immune donors into sublethally irradiated recipients resulted in a greater than 250-fold reduction in RacL11 in the lung. The elimination of both CD4+and CD8+T cells from the transferred cells abrogated clearance of RacL11, while the selective depletion of either subpopulation alone had little effect. These results suggested that both lymphocyte subpopulations contribute to viral clearance, with either subpopulation alone being sufficient.