Incorporation of iron into Tritrichomonas foetus cell compartments reveals ferredoxin as a major iron-binding protein in hydrogenosomes.
Incorporation of iron into Tritrichomonas foetus cell compartments reveals ferredoxin as a major iron-binding protein in hydrogenosomes.
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将铁掺入胎儿三毛滴虫细胞室表明铁氧还蛋白是氢化酶体中的主要铁结合蛋白。
作者：
P. Suchan;D. Vyoral;J. Petrák;R. Sut'ák;D. Rasoloson;E. Nohýnková;P. Doležal;J. Tachezy
铁的细胞内运输和其纳入细胞器中的过程在真核生物中知之甚少，在寄生原生生物中几乎一无所知。铁的运输在毛滴虫中特别令人感兴趣，毛滴虫具有氢化酶体而不是线粒体。氢体含有一组特定的FeS蛋白，其代谢功能完全取决于铁的获取。在这项工作中，铁纳入牛寄生虫胎儿三毛滴虫进行了监测。铁被有效地从（59）Fe-次氮基三乙酸中吸收，并在细胞质（88.9%）和氢化小体（总放射性的4.7%）中蓄积。使用原子吸收分光光度法，测得氢化酶体中异常高的稳态铁浓度[54.4+/-1.1 nmol Fe（mg蛋白质）（-1）]。细胞质中的铁浓度为13.4+/-0.5 nmol Fe（mg蛋白质）（-1）。采用非变性梯度PAGE对掺入的铁进行定性分析。大部分的（59）Fe在胞质溶胶中出现的不稳定铁池，这代表弱结合铁与化合物的分子量范围从5000至30000 Da。铁蛋白没有观察到Tt。胎儿，也没有在其他两个厌氧原生生物，溶组织内阿米巴和贾第鞭毛虫。分析Tt。胎儿氢化酶体显示出至少九种铁结合化合物，这在甲硝唑抗性突变体中是不存在的。主要的铁结合化合物被确定为肾上腺素氧还蛋白型的[2Fe-2S]铁氧还蛋白。
The intracellular transport of iron and its incorporation into organelles are poorly understood processes in eukaryotes and virtually unknown in parasitic protists. The transport of iron is of particular interest in trichomonads, which possess hydrogenosomes instead of mitochondria. The metabolic functions of hydrogenosomes, which contain a specific set of FeS proteins, entirely depend on iron acquisition. In this work the incorporation of iron into the cattle parasite Tritrichomonas foetus was monitored. Iron was efficiently taken up from (59)Fe-nitrilotriacetic acid and accumulated in the cytosol (88.9 %) and hydrogenosomes (4.7 % of the total radioactivity). Using atomic absorption spectrophotometry, an unusually high steady-state iron concentration in hydrogenosomes was determined [54.4+/-1.1 nmol Fe (mg protein)(-1)]. The concentration of iron in the cytosol was 13.4+/-0.5 nmol Fe (mg protein)(-1). Qualitative analysis of incorporated iron was performed using native gradient PAGE. The majority of the (59)Fe in the cytosol appeared as the labile-iron pool, which represents weakly bound iron associated with compounds of molecular mass ranging from 5000 to 30000 Da. Ferritin was not observed in Tt. foetus, nor in two other anaerobic protists, Entamoeba histolytica and Giardia intestinalis. Analysis of Tt. foetus hydrogenosomes showed at least nine iron-binding compounds, which were absent in metronidazole-resistant mutants. The major iron-binding compound was identified as [2Fe-2S] ferredoxin of the adrenodoxin type.
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厌氧原生生物阴道毛滴虫的氢酶体铁氧还蛋白的分子分析。
DOI：
10.1073/pnas.87.16.6097
发表时间：
1990
影响因子：
11.1
作者：
Johnson,PJ;d'Oliveira,CE;Gorrell,TE;Müller,M
通讯作者：
Müller,M
IscS 的系统发育表明，线粒体真核生物阴道毛滴虫和肠贾第鞭毛虫中的线粒体型铁硫簇组装。
影响因子：
10.7
作者：
Tachezy,J;Sánchez,LB;Müller,M
通讯作者：
Müller,M
甲硝唑耐药性阴道毛滴虫中铁氧还蛋白基因的转录减少。
DOI：
10.1073/pnas.89.10.4402
发表时间：
1992
影响因子：
11.1
作者：
Quon,DV;d'Oliveira,CE;Johnson,PJ
通讯作者：
Johnson,PJ
厌氧原虫胎儿三滴虫的氢核体铁氧还蛋白。
DOI：
--
发表时间：
1983
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Marczak,R;Gorrell,TE;Müller,M
通讯作者：
Müller,M