Sequences of the 5S rRNAs of Azotobacter vinelandii, Pseudomonas aeruginosa and Pseudomonas fluorescens with some notes on 5S RNA secondary structure.
Sequences of the 5S rRNAs of Azotobacter vinelandii, Pseudomonas aeruginosa and Pseudomonas fluorescens with some notes on 5S RNA secondary structure.
复制标题
维氏固氮菌、铜绿假单胞菌和荧光假单胞菌的 5S rRNA 序列以及 5S RNA 二级结构的一些注释。
DOI：
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发表时间：
1983
影响因子：
14.9
通讯作者：
R. de Wachter
中科院分区：
文献类型：
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作者：
E. Dams;A. Vandenberghe;R. de Wachter
最近发表的可用的5 S rRNA序列的比对表明，刚性碱基配对模式，指向所有5 S RNA的通用五螺旋二级结构的存在，可以叠加在这样的比对。对于少数物种，比对和碱基配对模式显示相对于大多数序列的失真。它们的5S RNA可能形成特殊的二级结构，或者可能只是在已发表的序列中存在错误。我们已经检查了这样一个例子，荧光假单胞菌，发现序列是错误的。校正的序列，以及相关物种棕色固氮菌和铜绿假单胞菌的序列，完全符合5 S RNA序列比对和五螺旋二级结构模型。有比较证据表明，在5S RNA二级结构的几个点上经常存在非标准碱基对。
Recently published alignments of available 5 S rRNA sequences have shown that a rigid base pairing pattern, pointing to the existence of a universal five-helix secondary structure for all 5 S RNAs, can be superimposed on such alignments. For a few species, the alignment and the base pairing pattern show distortions with respect to the large majority of sequences. Their 5 S RNAs may form exceptional secondary structures, or there may just be errors in the published sequences. We have examined such a case, Pseudomonas fluorescens, and found the sequence to be in error. The corrected sequence, as well as those of the related species Azotobacter vinelandii and Pseudomonas aeruginosa, fit perfectly in the 5 S RNA sequence alignment and in the five-helix secondary structure model. There exists comparative evidence for the frequent presence of non-standard base pairs at several points of the 5 S RNA secondary structure.