Application of the 6-aminoquinolyl-N-hydroxysccinimidyl carbamate (AQC) reagent to the RP-HPLC determination of amino acids in infant foods
Application of the 6-aminoquinolyl-N-hydroxysccinimidyl carbamate (AQC) reagent to the RP-HPLC determination of amino acids in infant foods
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DOI：
10.1016/j.jchromb.2005.12.002
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发表时间：
2006-02-02
影响因子：
3
通讯作者：
Farré, R
中科院分区：
文献类型：
--
作者：
Bosch, L;Alegría, A;Farré, R
对6-氨基喹啉-N-羟基琥珀酰亚胺氨基甲酸酯（AQC）柱前衍生化方法进行了验证，以通过RP-HPLC-荧光检测（λ激发波长250 nm，λ发射波长395 nm）测定乳-谷类婴儿食品中的氨基酸含量。分析参数：测定了线性（0.0025-0.2 mM）、方法精密度（0.2-3.5%变异系数）、准确度（衍生化：86-106%平均回收率和方法：88.3-118.2%平均回收率）以及检测限（0.016-0.367 μ M）和定量限（0.044-1.073 μ M）。谷氨酸，脯氨酸和亮氨酸是最丰富的氨基酸，而最低的含量对应于酪氨酸和半胱氨酸。(c)2005 Elsevier B. V.保留所有权利。
The validation of a pre-column derivatization procedure with 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate (AQC) to the determination of the amino acid content by RP-HPLC with fluorescence detection (lambda excitation 250 nm, lambda emission 395 nm) in milk-cereal based infant foods was carried out. The analytical parameters: linearity (0.0025-0.2 mM), precision of the method (0.2-3.5% variation coefficients), accuracy (derivatization: 86-106% average recovery and method: 88.3-118.2% average recovery) and the limits of detection (0.016-0.367 mu M) and quantification (0.044-1.073 mu M) were determined. Glutamic acid, proline and leucine were the most abundant amino acid whereas the lowest contents corresponded to tyrosine and cysteine. (c) 2005 Elsevier B.V. All rights reserved.