Characterization and Validation of Radiotracer Kinetics Using the Langendorff Isolated Perfused Heart.
Characterization and Validation of Radiotracer Kinetics Using the Langendorff Isolated Perfused Heart.
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使用 Langendorff 离体灌注心脏进行放射性示踪剂动力学的表征和验证。
DOI：
10.1007/978-1-0716-3499-8_15
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发表时间：
2024
期刊：
影响因子：
--
通讯作者：
Baark F
中科院分区：
文献类型：
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作者：
Baark F
Langendorff离体灌注心脏是一种生理相关和可控的离体模型，非常适合表征和验证用于广泛分子成像应用的新型放射性示踪剂。它允许监测首过示踪剂摄取动力学，无论是作为一个团注或作为一个连续输注在跳动的心肌组织与心脏工作负荷，灌注，能量底物输送和组成方面的高度实验控制，和药物共同管理。它提供的放射性示踪剂药代动力学数据不受诸如脱靶示踪剂代谢等混杂因素的污染，并且作为非成像技术，可以以非常高的时间分辨率获取时间活性曲线。在这一章中，我们描述了建立和使用Langendorff离体灌注心脏评估新的放射性示踪剂的基本原则和实践，并概述了他们的潜力，建模相关的心血管疾病的病理生理条件。
The Langendorff isolated perfused heart is a physiologically relevant and controllable ex vivo model well suited to characterizing and validating novel radiotracers for a wide range of molecular imaging applications. It allows the monitoring of first pass tracer uptake kinetics either as a bolus injection or as a continuous infusion in beating myocardial tissue with a high degree of experimental control in terms of cardiac workload, perfusion, energy substrate delivery and composition, and drug co-administration. The radiotracer pharmacokinetic data that it provides is not contaminated by confounding factors such as off-target tracer metabolism, and as a non-imaging technique, time activity curves can be acquired with very high temporal resolution. In this chapter, we describe the basic principles and practice for setting up and using Langendorff isolated perfused hearts for the assessment of novel radiotracers and outline their potential for modeling pathophysiological conditions relevant to cardiovascular disease.