Identification of a G1-S-phase-regulated region in the human thymidine kinase gene promoter.
Identification of a G1-S-phase-regulated region in the human thymidine kinase gene promoter.
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人胸苷激酶基因启动子中 G1-S 期调节区的鉴定。
DOI：
10.1128/mcb.10.7.3834-3837.1990
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发表时间：
1990
影响因子：
5.3
通讯作者：
Conrad,SE
中科院分区：
文献类型：
--
作者：
Roehl,HH;Conrad,SE
我们已经确定了人胸苷激酶基因启动子中的一个调控区。构建了一组启动子缺失突变体，将其与细菌新霉素抗性基因连接，并稳定地导入Rat3细胞。结果表明，帽点上游的135-67个碱基对之间的区域是将G1-S相调控传递到连锁新近系所必需的区域。此外，引物延伸分析表明，在G1期和S期细胞以及所测试的各种缺失突变体中都使用了相同的转录起始点。
We have identified a regulatory region in the human thymidine kinase gene promoter. A set of promoter deletion mutants was constructed, linked to the bacterial neomycin resistance gene, and stably transfected into Rat3 cells. It was shown that the region between 135 and 67 base pairs upstream of the cap site is required for conveying G1-S-phase regulation to the linkedneogene. In addition, primer extension assays demonstrated that the same transcriptional start sites were used in G1and S-phase cells and in the various deletion mutants tested.