VIP immunoreactivity in human aqueous humor.
VIP immunoreactivity in human aqueous humor.
复制标题
人房水中的 VIP 免疫反应性。
DOI：
10.1080/02713680490908715
复制
发表时间：
2005
期刊：
影响因子：
--
通讯作者：
Higginbotham,Eve
中科院分区：
文献类型：
--
作者：
Koh,Shay-Whey;Rutzen,Allan;Coll,Timothy;Hemady,Ramzi;Higginbotham,Eve
目的：证明从白内障或青光眼手术中收集的人房水中存在血管活性肠肽（VIP）免疫反应分子，并确定负责VIP免疫反应的分子团。方法：对9例白内障患者和8例青光眼手术患者在角膜缘结膜瓣形成、部分巩膜瓣剥离和丝裂霉素- c应用后，采用静脉滴注法采集房水标本。用放射免疫法分析房水标本，测定VIP免疫反应性水平。收集10例白内障患者的标本，采用Western blot分析VIP免疫反应性。结果：白内障和青光眼患者房水VIP免疫反应水平差异有统计学意义，分别为610±160和260±64 pg VIP/ml (p = 0.03)，而供体年龄与VIP免疫反应水平无相关性。检测到VIP的免疫反应性为单分子，分子量为9000。结论：影响房水中VIP免疫反应水平的因素是患者的疾病状况和手术治疗方法，以及在房水采集前立即进行的眼部手术，而不是患者的年龄。9000 Da VIP免疫反应分子与真正的（3326 Da） VIP之间的关系仍有待研究。
Purpose: To demonstrate the presence of vasoactive intestinal peptide (VIP)-immunoreactive molecule in the human aqueous humor collected from eyes undergoing either cataract or glaucoma surgeries and to identify the masses of molecules responsible for the VIP immunoreactivity.Methods: Aqueous humor specimens were collected by parencentesis from nine cataract patients and also from eight patients undergoing glaucoma surgery following the creation of the limbal based conjunctival flap, partial dissection of the scleral flap, and application of mitomycin-C. The aqueous humor specimens were analyzed by radioimmunoassay to determine the level of VIP immunoreactivity. Specimens from 10 other cataract patients were pooled and analyzed for VIP immunoreactivity by Western blot analysis.Results: Levels of VIP immunoreactivity in aqueous humor of cataract and glaucoma patients were significantly different and were 610 ± 160 and 260 ± 64 pg VIP/ml, respectively (p = 0.03), while there was no correlation between the donor age and the level of VIP immunoreactivity. VIP immunoreactivity was detected as a single molecule with a molecular weight of 9000.Conclusions: The disease status and the treatments of the eye that led to surgery and procedures applied to the eye immediately before aqueous humor collection, but not the age of the patients, affected the level of VIP immunoreactivity in the aqueous humor. The relationship between the 9000 Da VIP-immunoreactive molecule and the authentic (3326 Da) VIP remains to be studied.