FORMATION OF EPIDERMIS BY SERIALLY CULTIVATED HUMAN EPIDERMAL-CELLS TRANSPLANTED AS AN EPITHELIUM TO ATHYMIC MICE
FORMATION OF EPIDERMIS BY SERIALLY CULTIVATED HUMAN EPIDERMAL-CELLS TRANSPLANTED AS AN EPITHELIUM TO ATHYMIC MICE
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DOI：
10.1097/00007890-198004000-00010
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发表时间：
1980-01-01
期刊：
影响因子：
6.2
通讯作者：
GREEN, H
中科院分区：
文献类型：
--
作者：
BANKSSCHLEGEL, S;GREEN, H
通过接种解聚细胞的悬浮液，使人表皮细胞在表面培养物中连续生长。单个细胞生长成集落，集落融合形成融合上皮。将上皮作为一个单元分离并移植到在无胸腺小鼠中制备的移植床上。这些移植物形成了完整的表皮和角质层。虽然它们的面积大大减少，但移植物在移植后长达108天仍保持健康。人类起源的移植物被证明与物种特异性抗血清的前体蛋白的交联包膜。
Human epidermal cells were grown serially in surface culture by inoculation of suspensions of disaggregated cells. Single cells grew into colonies and the colonies fused to make a confluent epithelium. The epithelium was detached as a unit and transplanted onto a graft bed prepared in athymic mice. Such grafts formed epidermis complete with stratum corneum. Although they became considerably reduced in area, the grafts remained healthy for as long as 108 days after grafting. The human origin of the grafts was demonstrated with a species-specific antiserum to a precursor protein of the cross-linked envelope.