Microgravity induces proteomics changes involved in endoplasmic reticulum stress and mitochondrial protection.
Microgravity induces proteomics changes involved in endoplasmic reticulum stress and mitochondrial protection.
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DOI：
10.1038/srep34091
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发表时间：
2016-09-27
影响因子：
4.6
通讯作者：
Bowles DE
中科院分区：
文献类型：
--
作者：
Feger BJ;Thompson JW;Dubois LG;Kommaddi RP;Foster MW;Mishra R;Shenoy SK;Shibata Y;Kidane YH;Moseley MA;Carnell LS;Bowles DE
在地球上，生物系统是为了应对环境压力而进化的，这种相互作用是由包括重力在内的物理力决定的。没有重力是一个极端的压力源，它对生物系统的影响是不明确的。长时间处于最小重力（微重力）条件下的宇航员会经历一系列生物学变化，包括心血管功能的扰动。我们推测，在微重力下心脏功能的生理扰动可能是心肌细胞的细胞环境内的分子和细胞器动力学改变的结果。我们使用了一种基于质谱的方法相结合，比较了848和196蛋白质的相对丰度和周转率，分别在大鼠新生心肌细胞暴露于模拟微重力或正常重力。对这些数据的基因功能富集分析表明，线粒体、核糖体和内质网的蛋白质含量和功能在微重力条件下受到不同程度的调节。我们通过实验证实，在微重力下蛋白质合成减少，而细胞凋亡，细胞活力和蛋白质降解基本上不受影响。这些数据支持我们的结论，即在微重力心肌细胞试图维持线粒体的稳态，在蛋白质合成的代价。对这种应激的总体反应可能最终导致心肌萎缩。
On Earth, biological systems have evolved in response to environmental stressors, interactions dictated by physical forces that include gravity. The absence of gravity is an extreme stressor and the impact of its absence on biological systems is ill-defined. Astronauts who have spent extended time under conditions of minimal gravity (microgravity) experience an array of biological alterations, including perturbations in cardiovascular function. We hypothesized that physiological perturbations in cardiac function in microgravity may be a consequence of alterations in molecular and organellar dynamics within the cellular milieu of cardiomyocytes. We used a combination of mass spectrometry-based approaches to compare the relative abundance and turnover rates of 848 and 196 proteins, respectively, in rat neonatal cardiomyocytes exposed to simulated microgravity or normal gravity. Gene functional enrichment analysis of these data suggested that the protein content and function of the mitochondria, ribosomes, and endoplasmic reticulum were differentially modulated in microgravity. We confirmed experimentally that in microgravity protein synthesis was decreased while apoptosis, cell viability, and protein degradation were largely unaffected. These data support our conclusion that in microgravity cardiomyocytes attempt to maintain mitochondrial homeostasis at the expense of protein synthesis. The overall response to this stress may culminate in cardiac muscle atrophy.
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影响因子：
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作者：
Haynes, Cole M.;Ron, David
通讯作者：
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影响因子：
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通讯作者：
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