Anti-flagellin antibody responses elicited in mice orally immunized with attenuated Salmonella enterica serovar Typhimurium vaccine strains.
Anti-flagellin antibody responses elicited in mice orally immunized with attenuated Salmonella enterica serovar Typhimurium vaccine strains.
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DOI：
10.1590/s0074-02762008000600017
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发表时间：
2008-09
影响因子：
2.8
通讯作者：
L. Massis;Cjm Braga;M. Sbrogio-Almeida;C. Lauand;Smc Newton;PE Klebba;Lcs Ferreira
中科院分区：
文献类型：
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作者：
L. Massis;Cjm Braga;M. Sbrogio-Almeida;C. Lauand;Smc Newton;PE Klebba;Lcs Ferreira
在本研究中，我们研究了鞭毛蛋白特异性血清（IgG）和粪便（伊加）抗体反应引起的BALB/c小鼠与减毒沙门氏菌血清型鼠伤寒（S.表达1相（FliCi）、2相（FljB）或不表达内源性鞭毛蛋白的鼠伤寒沙门氏菌（Typhimurium）SL 3261菌株。本文报道的数据表明，用重组S.鼠伤寒菌株对FliCi、FljB或与FliCi基因融合的异源B细胞表位不产生显著的全身或分泌抗体应答。这些发现对于那些对利用鞭毛蛋白作为由减毒沙门氏菌介导的异源抗原的分子载体感兴趣的人特别相关。鼠伤寒菌株。
In the present study we investigated the flagellin-specific serum (IgG) and fecal (IgA) antibody responses elicited in BALB/c mice immunized with isogenic mutant derivatives of the attenuated Salmonella enterica serovar Typhimurium (S. Typhimurium) SL3261 strain expressing phase 1 (FliCi), phase 2 (FljB), or no endogenous flagellin. The data reported here indicate that mice orally immunized with recombinant S. Typhimurium strains do not mount significant systemic or secreted antibody responses to FliCi, FljB or heterologous B-cell epitopes genetically fused to FliCi. These findings are particularly relevant for those interested in the use of flagellins as molecular carriers of heterologous antigens vectored by attenuated S. Typhimurium strains.