Acetyl-l-Carnitine Ameliorates Caerulein-induced Acute Pancreatitis in Rats
Acetyl-l-Carnitine Ameliorates Caerulein-induced Acute Pancreatitis in Rats
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DOI：
10.1111/j.1742-7843.2009.00399.x
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发表时间：
2009-07-01
影响因子：
3.1
通讯作者：
Hamada, Farid M. A.
中科院分区：
文献类型：
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作者：
Arafa, Hossam M. M.;Hemeida, Ramadan A. M.;Hamada, Farid M. A.
在本研究中，我们已经解决了可能的保护作用，乙酰-L-肉毒碱在雨蛙素诱导的急性胰腺炎在雄性瑞士白化病大鼠。急性胰腺炎的范例是通过挑战动物与一个超大剂量的雨蛙肽（20 μ g/kg，SC），每小时间隔四次。雨蛙肽诱导的急性胰腺炎，其特征在于形态学和生化。严重水肿伴胰腺相对重量显著增加、腺泡显著萎缩伴腺泡间隙增加、空泡化和广泛白细胞浸润是胰腺炎表型的诊断特征。证实该模型的生化试验组合包括血浆淀粉酶和脂肪酶活性增加、钙水平以及胰腺酶髓过氧化物酶和谷胱甘肽-S-转移酶活性增加，以及胰腺一氧化氮和丙二醛含量增加和胰腺谷胱甘肽水平降低。在雨蛙肽激发前连续7天给予乙酰-L-肉毒碱（200 mg/kg，IP）可减轻急性胰腺炎的所有组织学和生化表现。这些结果表明，肉毒碱酯在这种小鼠急性胰腺炎模型中可能通过调节氧化剂/抗氧化剂平衡，除了调节髓过氧化物酶和一氧化氮系统，这是参与炎症级联反应，最常与疾病的可能的保护作用。
In the present study, we have addressed the possible protective role of acetyl-l-carnitine in caerulein-induced acute pancreatitis in male Swiss albino rats. Acute pancreatitis paradigm was developed by challenging animals with a supramaximal dose of caerulein (20 mu g/kg, SC) four times at hourly intervals. Caerulein induced acute pancreatitis that was well-characterized morphologically and biochemically. Severe oedema with marked increased relative pancreatic weight, marked atrophy of acini with increased interacinar spaces, vacuolization, and extensive leucocytic infiltration were diagnostic fingerprints of the pancreatitis phenotype. A biochemical test battery that confirmed the model comprised increased plasma amylase and lipase activities, calcium levels as well as increased pancreatic enzymatic myeloperoxidase and glutathione-S-transferase activities, beside increased pancreatic contents of nitric oxide and malondialdehyde and reduced pancreatic glutathione level. Prior administration of acetyl-l-carnitine (200 mg/kg, IP) for seven consecutive days ahead of caerulein challenge alleviated all the histological and biochemical manifestations of acute pancreatitis. These results suggest a possible protective role of the carnitine ester in such a murine acute pancreatitis model probably via regulation of the oxidant/antioxidant balance, beside modulation of the myeloperoxidase and nitric oxide systems, which are involved in the inflammatory cascade that most often associate the disease.