E plus subgroup PPR protein defective kernel 36 is required for multiple mitochondrial transcripts editing and seed development in maize and Arabidopsis
E plus subgroup PPR protein defective kernel 36 is required for multiple mitochondrial transcripts editing and seed development in maize and Arabidopsis
复制标题
玉米和拟南芥中多个线粒体转录本编辑和种子发育需要 E+ 亚组 PPR 蛋白缺陷籽粒 36
DOI：
10.1111/nph.14507
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发表时间：
2017
期刊：
影响因子：
9.4
通讯作者：
Song Rentao
中科院分区：
文献类型：
--
作者：
Wang Gang;Zhong Mingyu;Shuai Bilian;Song Ji;ong;Zhang Jie;Han Liang;Ling Huiling;Tang Yuanping;Wang Guifeng;Song Rentao
线粒体是半自主的细胞器，是细胞的动力源。由五肽重复序列(PPR)蛋白质引导的植物线粒体RNA编辑是能量产生的关键。我们鉴定了一种玉米籽粒缺陷突变体36，它产生小而塌陷的籽粒，导致胚胎和/或幼苗死亡。种子灌浆指数36严重受损，与胚乳转移组织中观察到的缺陷一致。定位克隆表明，编码线粒体靶向的E+亚群PPR蛋白的DEK36是线粒体RNA编辑所必需的，在atp4-59、nad7-383和ccmFN-302处，导致线粒体复合体I、复合体III和复合体IV指数36的活性降低。在DEK36处，拟南芥同源基因失去功能会导致胚胎和胚乳发育停滞，导致胚胎损害。重要的是，包括cmFN1、ccmFN2和rps12在内的所有编辑位点在at_dek36中的效率都显著降低，这可能是由于它们的RNA稳定性受损所致。这些结果表明，DEK36同源对在玉米和拟南芥中都是胚胎和胚乳发育所必需的，但在调节其线粒体靶标的RNA代谢方面具有不同的功能。
Mitochondria are semi‐autonomous organelles that are the powerhouse of the cells. Plant mitochondrial RNA editing guided by pentatricopeptide repeat (PPR) proteins is essential for energy production.We identify a maize defective kernel mutantdek36, which produces small and collapsed kernels, leading to embryos and/or seedlings lethality. Seed filling indek36is drastically impaired, in line with the defects observed in the organization of endosperm transfer tissue. Positional cloning reveals thatDEK36, encoding a mitochondria‐targeted E+ subgroup PPR protein, is required for mitochondrial RNA editing atatp4‐59,nad7‐383 andccmFN‐302, thus resulting in decreased activities of mitochondrial complex I, complex III and complex IV indek36.Loss‐of‐function of its Arabidopsis ortholog At DEK36 causes arrested embryo and endosperm development, leading to embryo lethality.At_dek36also has RNA editing defects inatp4,nad7,ccmFN1andccmFN2, but at the nonconserved sites. Importantly, efficiency of all editing sites inccmFN1,ccmFN2andrps12is severely decreased inAt_dek36, probably caused by the impairment of their RNA stabilization.These results suggest that the DEK36 orthologue pair are essential for embryo and endosperm development in both maize and Arabidopsis, but through divergent function in regulating RNA metabolism of their mitochondrial targets.