Polymorphisms of phosphate acquisition parameters in barley (Hordeum vulgare) landraces:: Secreted acid phosphatase and milieu acidification of roots after germination in vitro
Polymorphisms of phosphate acquisition parameters in barley (Hordeum vulgare) landraces:: Secreted acid phosphatase and milieu acidification of roots after germination in vitro
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DOI：
10.1080/01448765.2001.9754899
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发表时间：
2001-01-01
影响因子：
1.5
通讯作者：
Manninen, ML
中科院分区：
文献类型：
--
作者：
Ahokas, H;Manninen, ML
酸性磷酸酶活性和酸化的缺磷发芽培养基，其中39个基因型的大麦，包括34芬兰地方品种和5个“全球”行，无菌生长7天显着差异。大麦之间的差异具有统计学显著性，范围为p = 0.072至3 x 10（-14）。平均酸性磷酸酶活性范围为548 - 4525 nMol对硝基苯酚磷酸mg蛋白（-1）h（-1）。在7天内Ht浓度的平均增加从0.7到71.6 μ Mol ml（-1）培养基不等。酸性磷酸酶活性与培养基中分泌的蛋白量高度显著相关（r（s）在0.326和0.944之间变化）。籽粒质量与培养基中的酸性（r（s）= -0.041）和酸性磷酸酶活性（r（s）= -0.086）均不相关。酸化和酸性磷酸酶活性之间的相关性各不相同，在总的地方品种样本中显著（r（s）= 0.392），但在来自地方品种群体的27个品系样本中不显著（r（s）= 0.068），在全球品系样本中不显著（r（s）=-0.315）。芬兰地方品种是基因型混合物。不同的基因型也可以通过它们的根相互作用。遗传变异是适应性的，这是由于土壤微生境的高度异质性和其他选择因素也随时间和季节而变化，特别是在过去的田间管理实践中。一些地方品种基因型酸化根环境，而一些分泌高磷酸酶活性，如图所示，因此也是这些特征的广泛遗传资源。在低投入或有机农业中，栽培基因型或栽培品种的合适混合物，一些具有高分泌酸性磷酸酶活性，另一些有效地酸化根环境，可以从微溶性磷酸盐中受益于植物营养。
Significant differences are shown both for acid phosphatase activities and acidification of phosphorus-deficient germination media, in which 39 genotypes of barley, comprising 34 Finnish landrace and five 'global' lines, were grown axenically for 7 days. The differences between barleys had statistical significances varying from p = 0.072 to 3 x 10(-14). Mean acid phosphatase activities varied from 548 to 4525 nMol p-nitrophenol phosphate mg protein(-1) h(-1). Mean increases in Ht concentration during the 7 days varied from 0.7 to 71.6 mu Mol ml(-1) of medium. Acid phosphatase activity highly significantly correlated with the protein amount secreted in the medium (r(s) varying in different items between 0.326 and 0.944). Grain mass did not correlate with either acidification (r(s) = -0.041) or acid phosphatase activity in the medium (r(s) = -0.086). Correlations between acidification and acid phosphatase activity varied, being significant in the total landrace sample (r(s) = 0.392), but non-significant in the sample of 27 lines from a landrace population (r(s) = 0.068) and in the sample of the global lines (r(s) = - 0.315). The Finnish landraces were genotype mixtures. Different genotypes could also interact by their roots. Genetic variation was adaptive due to the highly heterogeneous soil microsites and other selective factors that also varied temporally and seasonally, especially during the past field management practices.Complementation in phosphate acquisition between different genotypes in soil was apparent in the genotype mixtures: some landrace genotypes acidified the root environment, while some secreted a high phosphatase activity as shown here, hence also being wide genetic resources of these characteristics. in low-input or organic farming, cultivation of a suitable mixtures of genotypes or cultivars, some with high secreted acid phosphatase activity, the others efficiently acidifying the root environment, might benefit plant nutrition from the sparingly soluble phosphate.