A lentiviral expression system demonstrates that L* protein of Theiler's murine encephalomyelitis virus (TMEV) has an anti-apoptotic effect in a macrophage cell line
A lentiviral expression system demonstrates that L* protein of Theiler's murine encephalomyelitis virus (TMEV) has an anti-apoptotic effect in a macrophage cell line
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DOI：
10.1016/j.micpath.2005.01.005
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发表时间：
2005-05-01
影响因子：
3.8
通讯作者：
Sawada, M
中科院分区：
文献类型：
--
作者：
Himeda, T;Ohara, Y;Sawada, M
TMEV的DA亚群毒株在感染小鼠的CNS中持续存在并诱导脱髓鞘。病毒持续存在和脱髓鞘的机制仍不清楚。DA亚群菌株从多聚蛋白的框架外起始位点合成17 kDa的蛋白质，称为L*。先前使用突变病毒DAL*-1（其中L* AUG被ACG取代）的研究表明，L* 在巨噬细胞系P388 D1中具有抗凋亡作用。因此，我们建立了持续表达L* 的P388 D1细胞，以证实其在TMEV诱导的凋亡中的作用。L* 的抗凋亡活性可能在TMEV发病机制中起重要作用。(c)2005爱思唯尔有限公司保留所有权利。
DA subgroup strains of TMEV persist in the CNS of infected mice and induce demyelination. The mechanism(s) of virus persistence and demyelination remains unknown. DA subgroup strains synthesize a 17-kDa protein, called L*, from an initiation site out-of-frame with the polyprotein. The previous study using a mutant virus, DAL*-1 (in which the L* AUG is substituted by an ACG) showed that L* has an antiapoptotic effect in a macrophage cell line, P388D1. Therefore, we established P388D1 cells that continuatively express L*, in order to confirm its role in TMEV-induced apoptosis. The anti-apoptotic activity of L* may be important in TMEV pathogenesis. (c) 2005 Elsevier Ltd. All rights reserved.