A redox-based electrogenetic CRISPR system to connect with and control biological information networks
A redox-based electrogenetic CRISPR system to connect with and control biological information networks
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DOI：
10.1038/s41467-020-16249-x
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发表时间：
2020-05-15
影响因子：
16.6
通讯作者：
Bentley, William E.
中科院分区：
文献类型：
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作者：
Bhokisham, Narendranath;VanArsdale, Eric;Bentley, William E.
电子信息可以通过电极激活的氧化还原介体直接从微电子器件传输到细胞。这些传输由氧化还原响应启动子解码，其使得能够对生物功能进行用户指定的控制。在这里，我们通过建立电子eCRISPR信息交换管道来建立这种氧化还原通信模式。该系统充当生物信号处理器，放大信号接收并过滤生物噪声。我们电子放大细菌群体感应（QS）信号通过激活LasI，自诱导-1合酶。同样，我们通过抑制天然SoxRS介导的氧化应激反应调节子来过滤掉非预期的噪音。然后，我们在E.大肠杆菌和肠道沙门氏菌。最后，我们展示了基于eCRISPR的信息处理，该信息处理允许时空氧化还原命令的传输，然后由明胶封装的E.杆菌我们预计，氧化还原通信渠道将使生物混合微电子设备，可以改变我们的能力，以电子方式解释和控制生物功能。氧化还原反应性转录调节因子可以使用户指定的生物功能的电子控制。在这里，作者展示了使用氧化还原信号对CRISPRa和CRISPRi的电子控制。
Electronic information can be transmitted to cells directly from microelectronics via electrode-activated redox mediators. These transmissions are decoded by redox-responsive promoters which enable user-specified control over biological function. Here, we build on this redox communication modality by establishing an electronic eCRISPR conduit of information exchange. This system acts as a biological signal processor, amplifying signal reception and filtering biological noise. We electronically amplify bacterial quorum sensing (QS) signaling by activating LasI, the autoinducer-1 synthase. Similarly, we filter out unintended noise by inhibiting the native SoxRS-mediated oxidative stress response regulon. We then construct an eCRISPR based redox conduit in both E. coli and Salmonella enterica. Finally, we display eCRISPR based information processing that allows transmission of spatiotemporal redox commands which are then decoded by gelatin-encapsulated E. coli. We anticipate that redox communication channels will enable biohybrid microelectronic devices that could transform our abilities to electronically interpret and control biological function. Redox-responsive transcriptional regulators can enable user-specified electronic control over biological functions. Here the authors demonstrate electronic control of CRISPRa and CRISPRi using redox signalling.