Protection of surface layer protein from Enterococcus faecium WEFA23 against Listeria monocytogenes CMCC54007 infection by modulating intestinal permeability and immunity
Protection of surface layer protein from Enterococcus faecium WEFA23 against Listeria monocytogenes CMCC54007 infection by modulating intestinal permeability and immunity
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通过调节肠道通透性和免疫力，保护屎肠球菌 WEFA23 表面层蛋白免受单核细胞增生李斯特菌 CMCC54007 感染
DOI：
10.1007/s00253-021-11240-y
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发表时间：
2021-05-14
影响因子：
5
通讯作者：
Wei, Hua
中科院分区：
文献类型：
--
作者：
He, Yao;Yang, Qin;Wei, Hua
此前发现屎肠球菌WEFA23能有效抑制单核增生李斯特菌CMCC54007的粘附和定植，这可能与其表面层蛋白（SLP）密切相关。本研究系统研究了屎肠杆菌WEFA23的SLP对单核增生乳杆菌CMCC54007感染的保护作用。体外实验表明，SLP能有效抑制单核增生乳杆菌向Caco-2细胞系的内化，降低促炎因子和毒力因子mRNA水平，恢复被破坏的肠道屏障。用5 M LiCl排除粪肠杆菌WEFA23的SLP，体内实验表明SLP增加了小鼠的体重，降低了单核增生乳杆菌CMCC54007的死亡率和细胞计数。进一步研究表明，SLP对单核增生乳杆菌CMCC54007感染的保护作用是通过调节肠道通透性和免疫，即降低血清中异硫氰酸荧光素-葡聚糖(FITC)-葡聚糖，改善结肠受损结构，增加杯状细胞数量和结肠中TJ蛋白（Claudin-1、Occludin和ZO-1）的蛋白水平。在免疫方面，SLP降低肝脏CD4+和CD8+ T细胞数量，降低mRNA水平，降低促炎因子IL-6、IL-1β、IFN-γ、TNF-α和NO含量，恢复肝脏和脾脏结构。•粪肠杆菌SLP可抑制单核增生乳杆菌的内化和定植•粪肠杆菌SLP可改善宿主肠道屏障功能障碍•粪肠杆菌SLP可降低促炎因子和促炎细胞
Enterococcus faecium WEFA23 was previously found effectively against adherence and colonization of Listeria monocytogenes CMCC54007, which might be closely related to its surface layer protein (SLP). In this study, the protective of SLP of E. faecium WEFA23 against infection of L. monocytogenes CMCC54007 was systemically investigated. In vitro assay showed that SLP actively inhibited L. monocytogenes internalization into Caco-2 cell line, with decreasing mRNA level of pro-inflammation cytokines and virulence factors and restoring destroyed intestinal barrier. In vivo assay through excluding SLP of E. faecium WEFA23 by 5 M LiCl represented that SLP increased body weight, reduced mortality and cell counts of L. monocytogenes CMCC54007 in tissues of mice. Further researches showed that SLP protected against L. monocytogenes CMCC54007 infection by modulation of intestinal permeability and immunity, namely, it decreased fluorescein isothiocyanate (FITC)-Dextran in serum, ameliorated destroyed colon structure, and increased number of goblet cells and protein level of TJ protein (Claudin-1, Occludin, and ZO-1) in colon. For immunity, SLP decreased number of CD4+ and CD8+ T cells in liver, mRNA level, and content of pro-inflammatory factors IL-6, IL-1β, IFN-γ ,TNF-α, and NO, and restored the structure of liver and spleen. Key Points •SLP of E. faecium inhibited L. monocytogenes internalization and colonization •SLP of E. faecium ameliorated host intestinal barrier dysfunction •SLP of E. faecium decreased pro-inflammatory cytokines and cells