Transmembrane communication in cells chronically infected with measles virus
Transmembrane communication in cells chronically infected with measles virus
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麻疹病毒慢性感染细胞的跨膜通讯
作者：
D. Tyrrell;A. Ehrnst
研究了麻疹病毒血凝素和溶血素表面蛋白与细胞内病毒抗原的跨膜结合。利用兔麻疹病毒基质和核衣壳蛋白单特异性抗血清和人血凝素和溶血素蛋白双特异性抗血清，研究了这些蛋白在慢性麻疹病毒感染人Lu 106细胞系中的共盖作用。表面相关包膜组分的盖层伴随着基质和核衣壳蛋白的共盖层，后者主要定位于包膜内。这证明了表面相关包膜组分与细胞内麻疹病毒基质和核衣壳蛋白之间的跨膜通信。结果表明，病毒包涵体和与细胞膜相关的病毒蛋白之间存在联系。在细胞松弛素B（1—2微克/毫升）存在的情况下，基质蛋白的共盖层没有变化或略有增强，而核衣壳蛋白的共盖层减少，表明肌动蛋白丝可能介导了病毒核衣壳与细胞膜之间的通讯。
The transmembrane association of the measles virus hemagglutinin and hemolysin surface proteins with intracellular viral antigens was studied. Rabbit antisera monospecific for measles virus matrix and nucleocapsid proteins and a human antiserum containing specificities for both the hemagglutinin and hemolysin proteins were used to study the co-capping of these proteins in human Lu 106 cell-line, chronically infected with measles virus. Capping of the surface-associated envelope components was accompanied by co-capping of the matrix and nucleocapsid proteins, the latter being localized mainly within the inclusions. This demonstrated transmembrane communication between surface-associated envelope components and the intracellular measles virus matrix and nucleocapsid proteins. The results demonstrated the existence of a linkage between viral inclusions and viral proteins associated with cell membranes. In the presence of cytochalasin B (1--2 micrograms/ml), co-capping of the matrix protein was unchanged or slightly enhanced, whereas co-capping of the nucleocapsid protein decreased, indicating that actin filaments may mediate the communication between viral nucleocapsids and the cell membrane.