Growth kinetics and lactic acid production of Lactobacillus plantarum NRRL B-4496, L-acidophilus NRRL B-4495, and L-reuteri B-14171 in media containing egg white hydrolysates
Growth kinetics and lactic acid production of Lactobacillus plantarum NRRL B-4496, L-acidophilus NRRL B-4495, and L-reuteri B-14171 in media containing egg white hydrolysates
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DOI：
10.1016/j.lwt.2019.01.058
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发表时间：
2019-05-01
影响因子：
6
通讯作者：
Sathivel, Subramaniam
中科院分区：
文献类型：
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作者：
Solval, Kevin Mis;Chouljenko, Alexander;Sathivel, Subramaniam
本研究的目的是评估含有蛋清水解物 (EWH) 和/或干蛋清 (DEW) 的发酵培养基与标准 MRS 培养基对乳酸菌 (LAB) 植物乳杆菌 NRRL B-4496、嗜酸乳杆菌 NRRL B-449 和罗伊氏乳杆菌 B-14171 生长的性能。评估了用于生长 LAB 的四种发酵培养基：(1) 标准 MRS 培养基、(2) 无氮源的 MRS (MRSN)、(3) 带 EWH 的 MRSN (MRSN-EWH) 和 (4) 带 DEW 的 MRSN (MRSN-DEW)。将发酵培养基与 LAB 在 37 ℃ 下孵育 24 小时，并定期取样以获得生长曲线、pH 值和可滴定酸度。结果表明，与 DEW 相比，EWH 的游离氨基酸含量更高。苏氨酸和亮氨酸是 EWH 中发现的主要游离氨基酸。 MRSN-EWH在生物量产量、最大生长速率和可滴定酸度方面比MRS更能促进嗜酸乳杆菌NRRL B-449和罗伊氏乳杆菌B-14171的生长。对于植物乳杆菌 NRRL B-4496，MRS 和 MRSN-EWH 培养基比 MRSN 和 MRSN DEW 培养基支持更高的细胞生物量产量。这项研究表明，蛋清水解物可用于发酵培养基中，以促进益生菌乳酸菌的生长。
The objective of this study was to assess the performance of fermentation media containing egg white hydrolysates (EWH) and/or dried egg white (DEW) with standard MRS media on the growth of lactic acid bacteria (LAB) Lactobacillus plantarum NRRL B-4496, L. acidophilus NRRL B-449, and L. reuteri B-14171. Four fermentation media for growing LAB were evaluated: (1) standard MRS media, (2) MRS with no nitrogen source (MRSN), (3) MRSN with EWH (MRSN-EWH), and (4) MRSN with DEW (MRSN-DEW). The fermentation media were incubated with LAB at 37 degrees C for 24 h and periodically sampled to obtain growth curves, pH, and titratable acidity. The results showed that EWH had higher amounts of free amino acids compared to DEW. Threonine and leucine were the predominant free amino acids found in EWH. MRSN-EWH was better at promoting the growth of L. acidophilus NRRL B-449 and L. reuteri B-14171 in terms biomass production, maximum growth rate and titratable acidity than MRS. MRS and MRSN-EWH media supported greater cell biomass yields than MRSN and MRSN DEW for L. plantarum NRRL B-4496. This study showed that egg white hydrolysates can be used in fermentation media to enhance the growth of probiotic lactic acid bacteria.