AFM-based analysis of human metastatic cancer cells
AFM-based analysis of human metastatic cancer cells
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DOI：
10.1088/0957-4484/19/38/384003
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发表时间：
2008-09-24
期刊：
影响因子：
3.5
通讯作者：
Gimzewski, James K.
中科院分区：
文献类型：
--
作者：
Cross, Sarah E.;Jin, Yu-Sheng;Gimzewski, James K.
最近，癌细胞的生物力学，特别是刚度或弹性，已被确定为与癌细胞功能、粘附、运动、转化和侵袭相关的重要因素。我们报告的纳米力学反应的转移性癌细胞和良性间皮细胞从人体腔液使用原子力显微镜。在我们的初步研究（Cross等2007 Nat. Nanotechnol. 2780 -3），我们报告了患者来源的渗出细胞的生物物理性质，并解决了细胞形态对测量的细胞硬度的影响。使用细胞离心法，产生形态上无法区分的细胞，可以在1分钟内制备，并避免任何可能的文物，由于12小时的离体培养，我们发现，转移性肿瘤细胞比良性细胞软超过80%，分布比正常细胞窄6倍以上。与我们先前的研究一致，该研究基于离体生长和形态学特征产生了可区分的细胞群，我们的结果表明，仅形态学不太可能足以解释这两种细胞类型的弹性模量差异。此外，对从患者收集的肿瘤和正常细胞固有的非特异性细胞粘附的分析显示，与正常细胞的粘附相比，肿瘤细胞的表面粘附类似于低33%的粘附。我们的研究结果表明，基于生物力学的功能分析可能会为未来癌症的细胞学评估和诊断提供额外的平台。
Recently biomechanics of cancer cells, in particular stiffness or elasticity, has been identified as an important factor relating to cancer cell function, adherence, motility, transformation and invasion. We report on the nanomechanical responses of metastatic cancer cells and benign mesothelial cells taken from human body cavity fluids using atomic force microscopy. Following our initial study ( Cross et al 2007 Nat. Nanotechnol. 2 780-3), we report on the biophysical properties of patient-derived effusion cells and address the influence of cell morphology on measured cell stiffness. Using a cytocentrifugation method, which yields morphologically indistinguishable cells that can be prepared in 1 min and avoids any possible artifacts due to 12 h ex vivo culture, we find that metastatic tumor cells are more than 80% softer than benign cells with a distribution over six times narrower than that of normal cells. Consistent with our previous study, which yielded distinguishable cell populations based on ex vivo growth and morphological characteristics, our results show it is unlikely that morphology alone is sufficient to explain the difference in elastic moduli for these two cell types. Moreover, analysis of non- specific cell adhesion inherent to tumor and normal cells collected from patients show surface adhesion of tumor cells is similar to 33% less adhesive compared to that of normal cells. Our findings indicate that biomechanical- based functional analysis may provide an additional platform for cytological evaluation and diagnosis of cancer in the future.