Serotonin promotes the survival of cortical glutamatergic neurons in vitro
Serotonin promotes the survival of cortical glutamatergic neurons in vitro
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DOI：
10.1006/exnr.1997.6633
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发表时间：
1997-11-01
影响因子：
5.3
通讯作者：
Parnavelas, JG
中科院分区：
文献类型：
--
作者：
Dooley, AE;Pappas, IS;Parnavelas, JG
5-羟色胺(5-羟色胺；5-羟色胺)在大脑皮层的出现与细胞增殖、存活和分化等发育事件相吻合。我们通过检测体外培养的大鼠皮质祖细胞，验证了5-羟色胺在这些事件中发挥作用的假设。用溴脱氧尿嘧啶核苷掺入法，我们发现5-羟色胺对这些细胞的增殖没有影响，但细胞存活实验表明它促进了它们的存活。观察到的存活效应被5-HT2a/2c受体激动剂α-甲基-5-羟色胺模拟，并被5-HT2a受体拮抗剂Cinanserin阻断。使用末端转移酶缺口末端标记法，发现暴露于5-羟色胺的培养物中细胞死亡减少，这与存活率的增加是一致的。细胞特异性标记物的免疫组织化学分析显示，5-羟色胺的作用仅针对含谷氨酸的神经元群体，而不针对任何其他类型的皮质细胞。这些结果表明，5-羟色胺对发育中的皮质神经上皮细胞的分裂没有作用，而是对有丝分裂后的神经元起作用。(C)1997年学术出版社。
The appearance of 5-hydroxytryptamine (serotonin; 5-HT) in the cerebral cortex coincides with developmental events such as cell proliferation, survival, and differentiation. We tested the hypothesis that 5-HT plays a role in these events by examining rat cortical progenitor cells in vitro. Using bromodeoxyuridine incorporation we found that 5-HT did not affect the proliferation of these cells, but a cell survival assay indicated that it promoted their survival. The observed survival effect was mimicked by the 5-HT2a/2c receptor agonist alpha-methyl-5-HT and blocked by the 5-HT2a receptor antagonist cinanserin. Consistent with increased survival was the finding, using the terminal transferase nick end labeling method, of reduced cell death in cultures exposed to 5-HT. Immunohistochemical analysis with cell-specific markers revealed that the effect of 5-HT was directed specifically to the glutamate-containing neuronal population and not to any other cortical cell types. These results indicate that 5-HT does not exert its effects on dividing neuroepithelial cells in the developing cortex, but rather on postmitotic neurons. (C) 1997 Academic Press.