The innervation of the mystacial pad of the rat as revealed by PGP 9.5 immunofluorescence
The innervation of the mystacial pad of the rat as revealed by PGP 9.5 immunofluorescence
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PGP 9.5 免疫荧光显示大鼠肌垫的神经支配
DOI：
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发表时间：
1993
期刊：
影响因子：
--
通讯作者：
J. Arvidsson
中科院分区：
文献类型：
--
作者：
F. Rice;E. Kinnman;H. Aldskogius;O. Johansson;J. Arvidsson
用抗人蛋白基因产物(PGP)9.5免疫荧光技术研究了大鼠肌垫的神经支配。PGP 9.5是一种泛素羧基末端水解酶，广泛分布于神经元胞浆。这项技术揭示了所有以前已知的神经支配，以及各种各样的小口径轴突和一些以前没有观察到的大口径传入纤维的末梢。新发现的与触须-毛囊窦复合体有关的神经支配包括：(1)表皮脊网领内的细径、放射状突起；(2)环窦水平的间充质鞘离心部的细径、环状排列突起的松散网络；(3)间充质鞘和海绵窦小梁间的松散的中、细口径突起网络；(4)海绵窦间充质鞘内和靠近基底膜的环状排列突起的松散网络；(5)海绵窦上部间充质鞘的大口径传入纤维向海绵窦上方的间充质鞘发出致密的网状终末网络；(6)所有触干底部的真皮乳头均有细口径神经支配。在触须间皮肤中，在表皮中检测到密集分布的细径的个体和簇状轮廓。除了先前已知的神经支配外，默克尔末梢一直在警卫毛口的表皮中被观察到，偶尔在警卫毛颈周围发现松散的细口径轴突网络，细口径侧面经常与鹿茸毛相连。血管轮廓在整个真皮中被严重支配。还标记了支配乳头肌的面神经轴突和运动终板。眶下神经横断术除面神经支配外，其余均无神经支配。单侧切除颈上神经节消除了对真皮乳头的神经支配，但没有引起其他明显的减少。表观雪旺细胞、触须毛囊外根鞘的Merkel细胞、表观树突状细胞和/或朗格汉斯细胞也表达PGP 9.5样免疫荧光。神经切断后2周，PGP 9.5样免疫荧光沿着中至大口径轴突的通常路线持续存在，呈高度空泡化。新发现的神经支配的可能功能作用与先前发现的传入神经的作用一起被考虑。©1993 Wiley-Liss，Inc.
The innervation of the mystacial pad in the rat was investigated with the aid of antihuman protein gene product (PGP) 9.5 immunofluorescence. PGP 9.5 is ubiquitin carboxyl‐terminal hydrolase, which is distributed throughout neuronal cytoplasm. This technique revealed all previously known innervation as well as a wide variety of small‐caliber axons and some endings of large‐caliber afferents that had not been observed before. Newly revealed innervation affiliated with vibrissal‐follicle sinus complexes included (1) fine‐caliber, radially oriented processes in the epidermal rete ridge collar; (2) a loose network of fine‐caliber, circumferentially arrayed processes in the centrifugal part of the mesenchymal sheath at the level of the ring sinus; (3) a loose haphazard network of fine‐caliber and medium‐caliber processes in the mesenchymal sheath and among the trabeculae of the cavernous sinus; (4) a loose network of circumferentially arrayed processes within the mesenchymal sheath of the cavernous sinus and in close proximity to the basement membrane; (5) a dense network of reticular‐like endings provided by large‐caliber afferents to the mesenchymal sheath in the upper part of the cavernous sinus; and (6) fine‐caliber innervation to the dermal papilla at the base of all vibrissal shafts. In the intervibrissal skin, a dense distribution of fine‐caliber individual and clustered profiles was detected in the epidermis. In addition to previously known innervation, Merkel endings were consistently observed in the epidermis at the mouths of guard hairs, loose networks of fine‐caliber axons were found around the necks of occasional guard hairs, and fine‐caliber profiles were frequently affiliated with vellus hairs. Vascular profiles were heavily innervated throughout the dermis. Axons and motor end plates of the facial nerve innervation to papillary muscles also were labeled. Transection of the infraorbital nerve eliminated all but the facial nerve innervation. Unilateral removal of the superior cervical ganglion eliminated the innervation to the dermal papillae but caused no other noticeable reduction. PGP 9.5‐like immunofluorescence was also moderately expressed in apparent Schwann cells, in Merkel cells only in the external root sheath of vibrissal follicles, and in apparent dendritic and/or Langerhans cells usually located in the epidermis and occasionally in the follicles. PGP 9.5‐like immunofluorescence persisted in highly vacuolated profiles along the usual courses of medium to large‐caliber axons 2 weeks after nerve transection. The possible functional role of the newly discovered innervation is considered along with that of previously identified afferents. © 1993 Wiley‐Liss, Inc.
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影响因子：
2.5
作者：
SIMONS, DJ;CARVELL, GE
通讯作者：
CARVELL, GE
DOI：
10.3109/07367228709144624
发表时间：
1987
期刊：
Somatosensory research
影响因子：
--
作者：
J. Silverman;Lawrence Kruger
通讯作者：
J. Silverman;Lawrence Kruger
影响因子：
2.5
作者：
V. Shea;E. Perl
通讯作者：
V. Shea;E. Perl
影响因子：
2.5
作者：
W. Renehan;M. Jacquin;R. Mooney;R. Rhoades
通讯作者：
W. Renehan;M. Jacquin;R. Mooney;R. Rhoades
薄感觉传入纤维的形态特征：“伤害感受器”功能的新解释。
影响因子：
--
作者：
Kruger,L
通讯作者：
Kruger,L