Long-Lasting Effects of a PEGylated Mutant Cocaine Esterase (CocE) on the Reinforcing and Discriminative Stimulus Effects of Cocaine in Rats
Long-Lasting Effects of a PEGylated Mutant Cocaine Esterase (CocE) on the Reinforcing and Discriminative Stimulus Effects of Cocaine in Rats
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DOI：
10.1038/npp.2011.226
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发表时间：
2012-04-01
影响因子：
7.6
通讯作者：
Woods, James H.
中科院分区：
文献类型：
--
作者：
Collins, Gregory T.;Narasimhan, Diwahar;Woods, James H.
最近的诱变研究发现了一种突变型 G4C/S10C/T172R/G173Q 可卡因酯酶 (CCRQ CocE)，其体外作用持续时间为 440 天。尽管 CCRQ CocE 的体内作用持续时间是右侧的 30 倍，但观察到这些作用的显着抑制长达 72 小时。尽管 PEG-CCRQ CocE 也略微降低了哌醋甲酯和食物强化反应的发生率，但这些影响是短暂的、持久的。
Recent mutagenesis studies have identified a mutant G4C/S10C/T172R/G173Q cocaine esterase (CCRQ CocE) with an in vitro duration of action of 440 days. Although the in vivo duration of CCRQ CocE's action was 30-fold to the right, with significant inhibition of these effects observed for up to 72 h. Although PEG-CCRQ CocE also produced slight reductions in the rates of methylphenidate-and food-reinforced responding, these effects were short-lived, lasting