Two hepcidins from spotted scat (Scatophagus argus) possess antibacterial and antiviral functions in vitro
Two hepcidins from spotted scat (Scatophagus argus) possess antibacterial and antiviral functions in vitro
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斑点粪便（Scatophagus argus）中的两种铁调素在体外具有抗菌和抗病毒功能
DOI：
10.1016/j.fsi.2016.01.038
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发表时间：
2016-03-01
影响因子：
4.7
通讯作者：
Zhang, Junbin
中科院分区：
文献类型：
--
作者：
Gui, Lang;Zhang, Peipei;Zhang, Junbin
铁调素是一类富含半胱氨酸的小分子抗菌肽，在宿主免疫中起重要作用。本研究从斑（Scatophagus argus）中克隆了两个hepcidin基因，分别命名为SA-hepcidin 1和SA-hepcidin 2，并对SA-hepcidin基因的组织分布进行了研究。此外，合成成熟SA-铁调素肽，以允许在体外评估其抗微生物和抗病毒功能。SA-铁调素1属于HAMP 1类，并且在斑点粪便的所有测试组织中广泛表达，而SA-铁调素2属于HAMP 2类，并且仅存在于肝脏中。合成的SA-铁调素对革兰氏阳性菌和革兰氏阴性菌具有相似的抗菌活性水平;然而，SA-铁调素1的抗菌活性强于SA-铁调素2。用草鱼鳍（GCF）和丘疹上皮瘤（EPC）细胞对合成的SA-铁调素进行抗中华鲟弹状病毒（SCRV）和大口黑鲈呼肠孤病毒（MsReV）的活性测定。SA-hepcidin 2具有抗病毒活性，而SA-hepcidin 1没有。这项研究的结果表明，SA-hepcidins是斑点粪便免疫系统中重要的多功能蛋白质，参与抵抗各种病原体。(C)2016爱思唯尔有限公司版权所有
Hepcidins are small cysteine-rich antimicrobial peptides that play an important role in host immunity against pathogenic organisms. In this study, two hepcidins, SA-hepcidin1 and SA-hepcidin2, were cloned from spotted scat (Scatophagus argus), and the tissue distributions of SA-hepcidins were determined. In addition, mature SA-hepcidin peptides were synthesized to allow evaluation of their antimicrobial and antiviral functions in vitro. SA-hepcidinl belongs to the HAMP1 class and is widely expressed in all tested tissues from spotted scat, whereas SA-hepcidin2 belongs to the HAMP2 class and present only in the liver. The synthetic SA-hepcidins had similar levels of antibacterial activity against Gram-positive and Gram-negative bacteria; however, the antibacterial activity of SA-hepcidinl was stronger than that of SA-hepcidin2. The antiviral activities of the synthetic SA-hepcidins were assessed against Siniperca chuatsi rhabdovirus (SCRV) and largemouth bass Micropterus salmoides reovirus (MsReV) in epithelioma papulosum cyprini (EPC) and grass carp fin (GCF) cells. SA-hepcidin2 had antiviral activity, but SA-hepcidinl did not. The results of this study suggest that SA-hepcidins are important multifunctional proteins in the spotted scat immune system that are involved in resistance to various pathogens. (C) 2016 Elsevier Ltd. All rights reserved.