Comparison of the Sensititre YeastOne® and CLSI M38-A2 microdilution methods in determining the activity of nine antifungal agents against dermatophytes
Comparison of the Sensititre YeastOne® and CLSI M38-A2 microdilution methods in determining the activity of nine antifungal agents against dermatophytes
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DOI：
10.1111/myc.13272
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发表时间：
2021-05-18
期刊：
影响因子：
4.9
通讯作者：
Zhang, Furen
中科院分区：
文献类型：
--
作者：
Chang, Wenqian;Bao, Fangfang;Zhang, Furen
皮肤癣菌是引起人类浅表感染的最常见的真菌病原体，在世界范围内具有很高的患病率。这些感染的治疗是基于局部和全身抗真菌药物的使用。目的评价Sensititre YeastOne（R）测定9种抗真菌药物对皮肤癣菌的体外活性的能力。方法将Sensititre（R）测定法与参比法对阿尼芬净（ANID）、米卡芬净钠（MCF）、醋酸卡泊芬净（CAS）、5-氟胞嘧啶（5 FC）、泊沙康唑（PCZ）、结果Sensititre（R）法测定的MIC或MEC值普遍低于M38-A2法，而M38-A2法测定的MIC或MEC值则低于M38-A2法。9种抗真菌药两种方法的总EA值以5 FC最高（100%），其次为MCF（94.9%）、PCZ（84.8%）、AMB（67.1%）、FLZ（65.8%）、VCZ（63.3%）、ANID（29.1%）、ITZ（20.3%）和CAS（2.5%）。除ANID（97.4%）、MCF（95%）和PCZ（92.5%）外，所有药物的两种方法之间的总体CA均为100%。除VCZ和5 FC外，所有药物均观察到显著差异。M38-A2的GMIC（或GMEC）和MIC 90（或MEC 90）结果按递增顺序依次为：MCF、PCZ、VCZ、ANID、ITZ、CAS、AMB、FLZ和5 FC。结论Sensititre YeastOne（R）与皮肤真菌参考方法的EA较差;因此，M38-A2应继续作为皮肤真菌抗真菌药敏试验的参考程序。
Background Dermatophytes are the most common fungal pathogens causing superficial infections in humans with a high prevalence worldwide. The treatment of these infections is based on the use of topical and systemic antifungal agents. A convenient method with a high predictive value for testing the susceptibilities of dermatophytes is necessary.Objective To evaluate the ability of the Sensititre YeastOne (R) in testing the activity of nine antifungal agents against dermatophytes.Methods We compared Sensititre (R) with reference procedure for anidulafungin (ANID), micafungin sodium (MCF), caspofungin acetate (CAS), 5-fluorocytosine (5FC), posaconazole (PCZ), voriconazole (VCZ), itraconazole (ITZ), fluconazole (FLZ) and amphotericin B (AMB) against 79 dermatophyte isolates, the essential agreement (EA) and categorical agreement (CA) between the two methods were obtained.Results The MICs or MECs obtained by the Sensititre (R) were usually lower than those obtained by the M38-A2. The overall EA between the two methods of nine antifungals was best for 5FC (100%), followed by MCF (94.9%), PCZ (84.8%), AMB (67.1%), FLZ (65.8%), VCZ (63.3%), ANID (29.1%), ITZ (20.3%) and CAS (2.5%). The overall CA between the two methods for all drugs was 100% except for ANID (97.4%), MCF (95%) and PCZ (92.5%). Substantial discrepancies were observed with all drugs except for VCZ and 5FC. The results of M38-A2 in terms of GMIC (or GMEC) and MIC90 (or MEC90) were, in increasing order, as follows: MCF, PCZ, VCZ, ANID, ITZ, CAS, AMB, FLZ and 5FC.Conclusions The Sensititre YeastOne (R) shows poor EA with the reference method for dermatophytes; therefore, M38-A2 should remain the reference procedure for antifungal susceptibility testing against dermatophytes.