SAP30, a component of the mSin3 corepressor complex involved in N-CoR-mediated repression by specific transcription factors
SAP30, a component of the mSin3 corepressor complex involved in N-CoR-mediated repression by specific transcription factors
复制标题
DOI：
10.1016/s1097-2765(00)80111-2
复制
发表时间：
1998-07-01
期刊：
影响因子：
16
通讯作者：
Eisenman, RN
中科院分区：
文献类型：
--
作者：
Laherty, CD;Billin, AN;Eisenman, RN
转录辅阻遏子mSin3在一个大型的多蛋白复合体中被发现，该复合体包含组蛋白去乙酰基酶HDAC1和HDAC2，以及至少五个紧密相关的多肽。我们克隆并鉴定了mSin3复合体的新成分SAP30。SAP30与mSin3结合，能够通过组蛋白去乙酰化酶介导转录抑制。SAP30还与N-COR辅阻遏子结合，并需要N-COR通过拮抗剂结合的雌激素受体和同源结构域蛋白RPX进行抑制，以及N-COR抑制POU结构域蛋白Pit-1的反式激活。然而，SAP30不是去连接的维甲酸受体或甲状腺激素受体介导的N-COR介导的抑制所必需的，这表明SAP30参与了mSin3-组蛋白脱乙酰酶复合体向N-COR辅阻遏子复合体的功能募集。
The transcriptional corepressor mSin3 is found in a large multiprotein complex containing the histone deacetylases HDAC1 and HDAC2, in addition to at least five tightly associated polypeptides. We have cloned and characterized a novel component of the mSin3 complex, SAP30. SAP30 binds to mSin3 and is capable of mediating transcriptional repression via histone deacetylases. SAP30 also binds the N-CoR corepressor and is required for N-CoR-mediated repression by antagonist-bound estrogen receptor and the homeodomain protein Rpx, as well as N-CoR suppression of transactivation by the POU domain protein Pit-1. However, SAP30 is not required for N-CoR-mediated repression by unliganded retinoic acid receptor or thyroid hormone receptor, suggesting that SAP30 is involved in the functional recruitment of the mSin3-histone deacetylase complex to a specific subset of N-CoR corepressor complexes.