ESCHERICHIA-COLI INTEGRATION HOST FACTOR BINDS TO SPECIFIC SITES IN DNA
ESCHERICHIA-COLI INTEGRATION HOST FACTOR BINDS TO SPECIFIC SITES IN DNA
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DOI：
10.1016/0092-8674(84)90478-1
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发表时间：
1984-01-01
期刊：
影响因子：
64.5
通讯作者：
NASH, HA
中科院分区：
文献类型：
--
作者：
CRAIG, NL;NASH, HA
E.大肠杆菌整合宿主因子（IHF）两者都直接参与噬菌体噬菌体。位点特异性重组并调节噬菌体和细菌基因的表达。使用保护免受核酸酶和化学攻击作为测定，IHF与DNA的相互作用进行了检查。IHF是特异性DNA结合蛋白，其与attP的三个不同区段相互作用，attP是噬菌体携带的重组位点。特异性IHF结合位点位于非att DNA中。几个非att的IHF结合位点，被确定为邻近的基因，其表达改变IHF突变体。通过对IHF保护序列的比较，提示IHF-DNA特异性相互作用的关键决定子包含在该序列中。图形 **。
E. coli integration host factor (IHF) both participates directly in phage .lambda. site-specific recombination and regulates the expression of phage and bacterial genes. Using protection from nuclease and chemical attack as an assay, the interaction of IHF with DNA was examined. IHF is a specific DNA binding protein that interacts with three distinct segments of attP, the recombination site carried by phage .lambda.. Specific IHF binding sites are located in non-att DNA. Several non-att IHF binding sites that were identified are adjacent to genes whose expression is altered in IHF mutants. From comparison of the sequences protected by IHF, it is suggested that the critical determinant in specific IHF-DNA interaction is contained in the sequence .**GRAPHIC**.