Molecular cloning and expression analysis of the cell-wall invertase gene family in rice (Oryza sativa L.)
Molecular cloning and expression analysis of the cell-wall invertase gene family in rice (Oryza sativa L.)
复制标题
DOI：
10.1007/s00299-004-0910-z
复制
发表时间：
2005-06-01
影响因子：
6.2
通讯作者：
Jeon, JS
中科院分区：
文献类型：
--
作者：
Cho, JI;Lee, SK;Jeon, JS
细胞壁转化酶 (CIN) 催化蔗糖水解成葡萄糖和果糖，通过质外体途径向库器官供应碳水化合物。为了研究水稻 (Oryza sativa L.) 中的 CIN 基因，我们通过搜索水稻序列数据库分离出与植物细胞壁转化酶蛋白氨基酸相似的 cDNA 克隆。概况分析表明，克隆的基因在各种器官中以独特的模式表达。例如，在未成熟种子中检测到 OsCIN1、OsCIN2、OsCIN4 和 OsCIN7 的转录本，而 OsCIN3 基因表达是花特异性的。对发育种子中表达的这些基因的进一步转录分析表明，OsCIN1、OsCIN2 和 OsCIN7 可能在蔗糖分配到胚和胚乳方面发挥重要作用。蔗糖是 CIN 的底物，可诱导离体叶片中 OsCIN1 转录本的积累，以及未成熟种子中 OsCIN2 转录本的积累，而用蔗糖处理的离体叶片中 OsCIN5 的水平显着下调。用稻瘟病（Magnaporthe grisea）作为生物应激源感染组织会增加 OsCIN1、OsCIN4 和 OsCIN5 的表达，表明这些基因可能参与代谢转换以抵抗病原体入侵。这些结果表明 OsCIN 基因发挥着多种作用，涉及代谢、生长、发育和应激反应的调节。
Cell-wall invertase (CIN) catalyzes the hydrolysis of sucrose into glucose and fructose for the supply of carbohydrates to sink organs via an apoplastic pathway. To study the CIN genes in rice (Oryza sativa L.), we isolated cDNA clones showing amino acid similarity to the plant cell wall invertase proteins from a search of rice sequence databases. Profile analyses revealed that the cloned genes are expressed in unique patterns in various organs. For example, transcripts of OsCIN1, OsCIN2, OsCIN4, and OsCIN7 were detected in immature seeds whereas OsCIN3 gene expression was flower-specific. Further transcript analysis of these genes expressed in developing seeds indicated that OsCIN1, OsCIN2, and OsCIN7 might play an important role involving sucrose partitioning to the embryo and endosperm. Sucrose, a substrate of CINs, induced the accumulation of OsCIN1 transcripts in excised leaves and OsCIN2 in immature seeds, while the level of OsCIN5 was significantly down-regulated in excised leaves treated with sucrose. Infecting the tissues with rice blast (Magnaporthe grisea) as a biotic stressor increased the expression of OsCIN1, OsCIN4, and OsCIN5, suggesting that these genes may participate in a switch in metabolism to resist pathogen invasion. These results demonstrate that OsCIN genes play diverse roles involving the regulation of metabolism, growth, development, and stress responses.