Supramolecular assembly composed of different mesogenic compounds possessing a ω-hydroxyalkyl unit exhibits suppressive effects on the A549 human lung cancer cell line
Supramolecular assembly composed of different mesogenic compounds possessing a ω-hydroxyalkyl unit exhibits suppressive effects on the A549 human lung cancer cell line
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DOI：
10.1039/c0md00126k
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发表时间：
2011-01-01
期刊：
影响因子：
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通讯作者：
Yoshizawa, Atsushi
中科院分区：
文献类型：
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作者：
Takahashi, Yuuka;Hazawa, Masaharu;Yoshizawa, Atsushi
研究了4-氰基-4′-（6-羟基己氧基）联苯（I-CN）、4-甲基氧基-4′-（6-羟基己氧基）联苯（I-OMe）及其二元混合物的抗肿瘤活性和介生性质。采用流式细胞术和Western blot技术对A549人肺癌细胞的生长、细胞周期分布和细胞信号通路进行研究。I-CN （2.5 μ M）和I-OMe （2.5 μ M）等摩尔混合物对A549人肺癌细胞增殖的抑制作用强于I-CN或I-OMe。该混合物不影响细胞对WI-38正常成纤维细胞的增殖。混合物的热致性和溶致性表明，在微摩尔量级的浓度下，I-CN和I-OMe之间发生分子聚集。这些结果表明，由不同分子组成的超分子组装在液晶分子的生物活性中起着重要作用。
We investigated the anti-tumor activity and mesogenic properties of 4-cyano-4'-(6-hydroxyhexyloxy)biphenyl (I-CN), 4-methyloxy-4'-(6-hydroxyhexyloxy)biphenyl (I-OMe) and their binary mixtures. The anti-tumor properties of the materials were investigated in A549 human lung cancer cells by assessing cell growth, cell cycle distribution and cell signaling pathways using flow cytometry and Western blot analysis. An equimolar mixture of I-CN (2.5 mu M) and I-OMe (2.5 mu M) exhibited stronger inhibition of cell proliferation in A549 human lung cancer cells than I-CN or I-OMe. The mixture did not affect cell proliferation against WI-38 normal fibroblasts. Both thermotropic and lyotropic mesogenic properties of the mixture indicate that molecular aggregation between I-CN and I-OMe occurs at a concentration of micromolar order. These results reveal that the supramolecular assembly composed of different molecules plays an important role in the biological activity of liquid-crystalline molecules.