Genotoxicity of di-butyl-phthalate and di-iso-butyl-phthalate in human lymphocytes and mucosal cells
Genotoxicity of di-butyl-phthalate and di-iso-butyl-phthalate in human lymphocytes and mucosal cells
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DOI：
10.1002/tcm.1007
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发表时间：
2001-01-01
期刊：
影响因子：
--
通讯作者：
Harréus, UA
中科院分区：
文献类型：
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作者：
Kleinsasser, NH;Wallner, BC;Harréus, UA
在先前使用彗星试验的研究中，邻苯二甲酸二丁酯（DBP）和邻苯二甲酸二异丁酯（DiBP）在人体上呼吸消化道粘膜细胞中的遗传毒性已被证明是广泛使用的增塑剂。此外，与非肿瘤患者相比，喉或口咽鳞状细胞癌患者的遗传毒性敏感性更高。其他作者已经证明了人体淋巴细胞中不同邻苯二甲酸酯的DNA损伤。本研究的目的是确定粘膜细胞或淋巴细胞对DBP及其异构体DiBP的遗传毒性敏感性之间是否存在相关性。应用单细胞微凝胶电泳技术（彗星试验）检测了132例人上呼吸消化道上皮细胞DNA链断裂。从非肿瘤患者和患有口咽鳞状细胞癌的患者的口咽收获人粘膜。同时收集喉鳞状细胞癌患者的喉粘膜。从外周血中分离外周淋巴细胞（n=49份标本）。研究的异生物质分别是DBP、DiBP和作为阳性对照的N '甲基-N'-硝基-N-亚硝基胍（MNNG）。对于统计分析，根据Pearson和Wilcoxon检验进行SPSS（TM）相关性分析。DBP和DiBP对上皮细胞和淋巴细胞有遗传毒性（P
The genotoxicity of phthalates, widely used plasticizers, has been shown previously for di-butyl-phthalate (DBP) and di-iso-butyl-phthalate (DiBP) in human mucosal cells of the upper aerodigestive tract in a previous study using the Comet assay. Furthermore, higher genotoxic sensitivities of patients with squamous cell carcinomas of either the larynx or the oropharynx compared to non-tumor patients were described. Other authors have demonstrated DNA damage by a different phthalate in human lymphocytes. It was the aim of the present study to determine whether there is a correlation between the genotoxic sensitivities to DBP and its isomer DiBP in either mucosal cells or lymphocytes. The single-cell microgel electrophoresis assay (Comet assay) was applied to detect DNA strand breaks in human epithelial cells of the upper aerodigestive tract (n=132 specimens). Human mucosa was harvested from the oropharynx in non-tumor patients and patients with squamous cell carcinomas of the oropharynx. Laryngeal mucosa of patients with laryngeal squamous cell carcinomas was harvested as well. Peripheral lymphocytes (n=49 specimens) were separated from peripheral blood. Xenobiotics investigated were DBP, DiBP, and N'methyl-N'-nitro-N-nitrosoguanidine (MNNG) as positive control, respectively. For statistical analysis, the SPSS (TM) correlation analysis according to Pearson and the Wilcoxon test were performed. Genotoxicity was found for DBP and DiBP in epithelial cells and lymphocytes (P