Polymorphism of Japanese isolates of the pinewood nematode, Bursaphelenchus xylophilus (Aphelenchida: Aphelenchoididae), at heat-shock protein 70A locus and the field detection of polymorphic populations
Polymorphism of Japanese isolates of the pinewood nematode, Bursaphelenchus xylophilus (Aphelenchida: Aphelenchoididae), at heat-shock protein 70A locus and the field detection of polymorphic populations
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DOI：
10.1303/aez.2007.247
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发表时间：
2007-05
影响因子：
1.3
通讯作者：
S. Takemoto;K. Futai
中科院分区：
文献类型：
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作者：
S. Takemoto;K. Futai
对29株松材线虫热休克蛋白70A基因(Hsp70A)进行了聚合酶链式反应-限制性片段长度多态(PCR-RFLP)分析。其中，两个已知的无毒分离物和五个强毒分离物产生的带型在两个分离物组内相同，但在两个分离物组之间不同。另一方面，在茨城县鹿玉市和日本千叶县久久库里滨海岸南部两个地理区域收集了野外种群。聚合酶链式反应-限制性片段长度多态性图像分析表明，部分群体同时具有这两种等位基因。等位基因在两个采样区的地理分布是随机的。根据这些结果，我们推测该无毒菌株可能对松材线虫的基因库有一定的贡献，尽管最近还没有从日本收集无毒种群的报道。
Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) pattern of heat-shock protein 70A gene (hsp70A) was examined for 29 isolates of the pinewood nematode. Among them, two known avirulent and five virulent isolates generated banding patterns identical within each of the two isolate groups but distinctive between the two. On the other hand, field populations were collected in two geographical areas, around Kasama City, Ibaraki Prefecture and the southern part of Kujukurihama coast, Chiba Prefecture, Japan. PCR-RFLP image analysis revealed that some of the populations possessed both of the two alleles. The geographical distribution of the alleles was random in the two sampling areas. On the basis of the results, we hypothesize that the avirulent strain might have a certain contribution to the gene pool of the pinewood nematode, although there has been no report on the collection of an avirulent population from Japan recently.