REACTIVE ABSORPTION OF OZONE BY AQUEOUS BIOMOLECULE SOLUTIONS - IMPLICATIONS FOR THE ROLE OF SULFHYDRYL COMPOUNDS AS TARGETS FOR OZONE
REACTIVE ABSORPTION OF OZONE BY AQUEOUS BIOMOLECULE SOLUTIONS - IMPLICATIONS FOR THE ROLE OF SULFHYDRYL COMPOUNDS AS TARGETS FOR OZONE
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DOI：
10.1006/abbi.1995.1009
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发表时间：
1995-01-10
影响因子：
3.9
通讯作者：
SIMA, PD
中科院分区：
文献类型：
--
作者：
KANOFSKY, JR;SIMA, PD
测定了各种生物分子溶液对臭氧的反应性吸收速率。在pH 7时，各种生物分子对臭氧的反应性大小顺序为：硫代硫酸盐>抗坏血酸盐>半胱氨酸近似蛋氨酸>谷胱甘肽。然后使用数学模型分析在各种条件下的反应吸收率，以估计各种臭氧-生物分子反应的反应速率常数。与臭氧-蛋氨酸反应速率常数相比，硫代硫酸盐、抗坏血酸盐、半胱氨酸和谷胱甘肽反应的相对速率常数分别为18 +/- 2、12 +/- 1、1.1 +/- 0.1和0.62 +/- 0.03。使用4 × 10（6）M（-1）s（-1）的臭氧-甲硫氨酸反应速率常数，硫代硫酸盐、抗坏血酸盐、半胱氨酸和谷胱甘肽的速率常数分别为7：2 × 10（7）、4.8 × 10（7）、4.4 × 10（6）和2.5 × 10（6）M（-1）s（-1）。使用色氨酸作为标准臭氧目标的竞争性研究与这些速率常数是一致的。与色氨酸相比，蛋氨酸、半胱氨酸和谷胱甘肽的相对臭氧反应速率常数分别为0.77 +/- 0.08、0.88 +/- 0.19和0.42 +/- 0.01。这些相对速率常数是指臭氧消耗而不是生物分子消耗，因此它们可以与从反应性吸收获得的相对速率常数进行比较。此外，各种不一致的文献中关于臭氧-半胱氨酸和臭氧-谷胱甘肽反应的速率进行了审查。(C)出版社：Academic Press
The rates of reactive absorption of ozone by various biomolecule solutions were measured. At pH 7, the ability of various biomolecules to reactively absorb ozone was in the following sequence: thiosulfate > ascorbate > cysteine approximate to methionine > glutathione. The rates of reactive absorption under a variety of conditions were then analyzed using a mathematical model in order to estimate the reaction rate constants for the various ozone-biomolecule reactions. Compared to the ozone-methionine rate constant, the relative rate constants for thiosulfate, ascorbate, cysteine, and glutathione reactions were 18 +/- 2, 12 +/- 1, 1.1 +/- 0.1, and 0.62 +/- 0.03, respectively. Using an ozone-methionine reaction rate constant of 4 x 10(6) M(-1) s(-1), the rate constants for thiosulfate, ascorbate, cysteine, and glutathione were 7:2 X 10(7), 4.8 X 10(7), 4.4 X 10(6), and 2.5 X 10(6) M(-1) s(-1), respectively. Competitive studies using tryptophan as a standard ozone target were consistent with these rate constants. Compared to tryptophan, the relative ozone reaction rate constants for methionine, cysteine, and glutathione were 0.77 +/- 0.08, 0.88 +/- 0.19, and 0.42 +/- 0.01, respectively. These relative rate constants refer to ozone consumption rather than biomolecule consumption, so they may be compared with the relative rate constants obtained from reactive absorption. In addition, various inconsistencies in the literature regarding the rates of the ozone-cysteine and the ozone-glutathione reactions were reviewed. (C) 1995 Academic Press, Inc.