Slow components of axonal transport: two cytoskeletal networks.
Slow components of axonal transport: two cytoskeletal networks.
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DOI：
10.1083/jcb.86.2.616
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发表时间：
1980-08
期刊：
影响因子：
--
通讯作者：
Lasek RJ
中科院分区：
文献类型：
--
作者：
Black MM;Lasek RJ
我们在豚鼠视网膜神经节细胞轴突中发现了两组缓慢移动的轴突运输蛋白 (4)。最慢的一组蛋白质，称为慢成分 a (SCa)，运输速率为 0.25 mm/d，由微管蛋白和神经丝蛋白组成。另一组缓慢运输的蛋白质称为慢速成分 b (SCb)，运输速率为 2-3 mm/d，由许多多肽组成，其中之一是肌动蛋白 (4)。我们对SCa和SCb的各个多肽的转运动力学的分析表明（a）SCa的多肽在视轴突中连贯地转运，（b）SCb的多肽也被连贯地转运但与SCa多肽完全分开，以及（c）SCa的多肽与包含SCb的多肽完全不同。我们将这些结果与我们的一般假设联系起来，即缓慢的轴突运输代表蛋白质结构复合物的运动。此外，有人提出SCa对应于微管-神经丝网络，SCb代表微丝网络以及与微丝复合的蛋白质的运输。
We have identified two slowly moving groups of axonally transported proteins in guinea pig retinal ganglion cell axons (4). The slowest group of proteins, designated slow component a (SCa), has a transport rate of 0.25 mm/d and consists of tubulin and neurofilament protein. The other slowly transported group of proteins, designated slow components b (SCb), has a transport rate of 2-3 mm/d and consists of many polypeptides, one of which is actin (4). Our analyses of the transport kinetics of the individual polypeptides of SCa and SCb indicate that (a) the polypeptides of SCa are transported coherently in the optic axons, (b) the polypeptides of SCb are also transported coherently but completely separately from the SCa polypeptides, and (c) the polypeptides of SCa differ completely from those comprising SCb. We relate these results to our general hypothesis that slow axonal transport represents the movements of structural complexes of proteins. Furthermore, it is proposed that SCa corresponds to the microtubule- neurofilament network, and that SCb represents the transport of the microfilament network together with the proteins complexed with microfilaments.