Protective Effects of Exogenous Hydrogen Sulfide on Neurons of Hippocampus in a Rat Model of Brain Ischemia
Protective Effects of Exogenous Hydrogen Sulfide on Neurons of Hippocampus in a Rat Model of Brain Ischemia
复制标题
外源性硫化氢对脑缺血模型大鼠海马神经元的保护作用
DOI：
10.1007/s11064-011-0502-6
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发表时间：
2011-10-01
影响因子：
4.4
通讯作者：
Zhang, Tao
中科院分区：
文献类型：
--
作者：
Li, Zhanyong;Wang, Yiyi;Zhang, Tao
许多研究证实了硫化氢在体外和/或体内缺血损伤中的细胞保护作用。本研究旨在探讨外源性硫化氢是否能减轻脑缺血所致的神经元损伤。以硫化氢供体(NaHS)为供体，腹腔注射NaHS(5.6 mg/kg/d)。采用行为学、电生理学、形态学和免疫组织化学方法观察外源性H_2S对缺血海马神经元的影响。结果表明，外源性硫化氢可显著改善脑缺血所致的空间学习记忆障碍(P<；P<；0.01)。外源性H_2S增强脑缺血大鼠海马区突触可塑性，抑制脑缺血后锥体细胞周围水肿和核萎缩，促进脑缺血后海马CA1区生长相关蛋白-43(GAP-43)的表达。提示硫化氢对脑缺血具有保护作用和治疗潜力。
Many studies have demonstrated the cytoprotective effects of hydrogen sulfide (H2S) in vitro and/or in vivo ischemic injury. The aim of the current study was to investigate whether exogenous H2S attenuates the neuronal injury induced by brain ischemia. As an H2S donor, sodium hydrosulfide (NaHS) was administered intraperitoneally (5.6 mg/kg/day, i.p.). The effects of exogenous H2S on neurons of ischemic hippocampus were examined by using measurement of behavior, electrophysiology, morphology and immunohistochemical staining, respectively. Our results showed that exogenous H2S significantly improved spatial learning and memory deficits induced by brain ischemia (P< 0.01). Exogenous H2S enhanced synaptic plasticity in the hippocampus of brain-ischemic rats, inhibited the edema around pyramidal neurons and the nuclear shrink induced by ischemia, and promoted the expression of growth-associated protein-43 (GAP-43) in the CA1 region of hippocampus post ischemia. The results suggest a protective effect and therapeutic potential of H2S in the treatment of brain ischemia.