A peptide derived from herpes simplex virus type 1 glycoprotein H: membrane translocation and applications to the delivery of quantum dots
A peptide derived from herpes simplex virus type 1 glycoprotein H: membrane translocation and applications to the delivery of quantum dots
复制标题
DOI：
10.1016/j.nano.2011.04.009
复制
发表时间：
2011-12-01
影响因子：
5.4
通讯作者：
Galdiero, Stefania
中科院分区：
文献类型：
--
作者：
Falanga, Annarita;Vitiello, Maria Teresa;Galdiero, Stefania
细胞膜对大多数不由活细胞主动输入的分子是不可渗透的，包括所有大分子，甚至是其理化性质阻止被动膜扩散的小分子。然而，最近，我们看到细胞内药物输送方法的发展日益复杂。细胞穿透肽（CPP）是一种短肽，据信可以通过穿透细胞膜进入细胞，它引起了人们的极大兴趣，希望能够增强基因治疗、疫苗开发和药物输送。然而，为了实现有效的细胞内递送，必须研究绕过内吞途径的进一步策略。我们报告了一种源自 I 型单纯疱疹病毒糖蛋白 gH 的新型肽分子，与现有的 CPP 相比，它能够穿过双层膜并将货物转运到细胞质中，具有新的特性。我们使用本身不会显着穿过膜双层的量子点作为货物分子。来自临床编辑：细胞穿透肽最近在优化基因治疗、疫苗开发和药物输送方面引起了极大的兴趣。本研究从这一观点出发，对源自单纯疱疹I型糖蛋白gH的肽进行了研究。 (C) 2011 Elsevier Inc. 保留所有权利。
Cell membranes are impermeable to most molecules that are not actively imported by living cells, including all macromolecules and even small molecules whose physiochemical properties prevent passive membrane diffusion. However, recently, we have seen the development of increasingly sophisticated methodology for intracellular drug delivery. Cell-penetrating peptides (CPPs), short peptides believed to enter cells by penetrating cell membranes, have attracted great interest in the hope of enhancing gene therapy, vaccine development and drug delivery. Nevertheless, to achieve an efficient intracellular delivery, further strategies to bypass the endocytotic pathway must be investigated. We report on a novel peptide molecule derived from glycoprotein gH of herpes simplex type I virus that is able to traverse the membrane bilayer and to transport a cargo into the cytoplasm with novel properties in comparison with existing CPPs. We use as cargo molecule quantum dots that do not significantly traverse the membrane bilayer on their own.From the Clinical Editor: Cell-penetrating peptides have recently attracted great interest in optimizing gene therapy, vaccine development and drug delivery. In this study, a peptide derived from glycoprotein gH of herpes simplex I is investigated from this standpoint. (C) 2011 Elsevier Inc. All rights reserved.