Detection of biological thiols based on a colorimetric method
Detection of biological thiols based on a colorimetric method
复制标题
DOI：
10.1631/jzus.b1500232
复制
发表时间：
2016-10
影响因子：
5.1
通讯作者：
Yuan-yuan Xu;Yang-yang Sun;Yu-juan Zhang;Chen-he Lu;J. Miao
中科院分区：
文献类型：
--
作者：
Yuan-yuan Xu;Yang-yang Sun;Yu-juan Zhang;Chen-he Lu;J. Miao
Biological thiols (biothiols), an important kind of functional biomolecules, such as cysteine (Cys) and glutathione (GSH), play vital roles in maintaining the stability of the intracellular environment. In past decades, studies have demonstrated that metabolic disorder of biothiols is related to many serious disease processes and will lead to extreme damage in human and numerous animals. We carried out a series of experiments to detect biothiols in biosamples, including bovine plasma and cell lysates of seven different cell lines based on a simple colorimetric method. In a typical test, the color of the test solution could gradually change from blue to colorless after the addition of biothiols. Based on the color change displayed, experimental results reveal that the percentage of biothiols in the embryonic fibroblast cell line is significantly higher than those in the other six cell lines, which provides the basis for the following biothiols-related study.中文概要目 的通过简单可靠的可视化检测方法评估牛血清及各细胞系中生物巯化物的含量。创新点基于银纳米颗粒形成的比色变化过程对牛血清及细胞中生物巯化物进行了检测。方 法将6 组不同的细胞系培养后进行裂解,其裂解产物分别与3,3′,5,5′-四甲基联苯胺(TMB)和硝酸银(AgNO3)的混合液室温孵育后,用紫外可见分光光度计测量细胞中生物巯化物的含量。结 论通过不同细胞系中生物巯化物含量的比对,证实胚胎成纤维细胞中生物巯化物的含量明显高于其他细胞。