Polyethylenimine (PEI) is a simple, inexpensive and effective reagent for condensing and linking plasmid DNA to adenovirus for gene delivery
Polyethylenimine (PEI) is a simple, inexpensive and effective reagent for condensing and linking plasmid DNA to adenovirus for gene delivery
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DOI：
10.1038/sj.gt.3300471
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发表时间：
1997-08-01
期刊：
影响因子：
5.1
通讯作者：
Cotten, M
中科院分区：
文献类型：
--
作者：
Baker, A;Saltik, M;Cotten, M
本文介绍了一种简便、廉价的将质粒DNA与腺病毒载体连接的方法。合成的聚阳离子聚乙烯亚胺用于将质粒DNA缩合成带正电荷的100 nm复合物。然后，这些PEI-DNA复合物通过与病毒六邻体上的负结构域的电荷相互作用结合到腺病毒颗粒上。由于使用了灭活的病毒，因此所得的转染复合物通过腺病毒感染途径将质粒DNA递送至细胞，而不受病毒基因表达的干扰。PN-DNA-腺病毒复合物显示出与采用链霉亲和素/生物素连接的更复杂的DNA病毒复合物相当的DNA递送，但不需要特殊试剂并且更容易制备。
A simple and inexpensive method of condensing and linking plasmid DNA to carrier adenovirus particles is described. The synthetic polycation polyethylenimine is used to condense plasmid DNA into positively charged 100 nm complexes. These PEI-DNA complexes are then bound to adenovirus particles through charge interactions with negative domains on the viral hexon. The resulting transfection complexes deliver plasmid DNA to cells by the adenovirus infectious route without interference from virus' gene expression because psoralen-inactivated virus is employed. The PN-DNA-adenovirus complexes display DNA delivery comparable to more sophisticated DNA virus complexes employing streptavidin/biotin linkage, but require no special reagents and are much easier to prepare.