Adherence of yeast and filamentous forms of Candida albicans to cultured enterocytes.
Adherence of yeast and filamentous forms of Candida albicans to cultured enterocytes.
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DOI：
10.1097/00003246-200203000-00030
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发表时间：
2002-03
影响因子：
8.8
通讯作者：
S. Wiesner;C. Bendel;D. Hess;S. Erlandsen;C. Wells
中科院分区：
文献类型：
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作者：
S. Wiesner;C. Bendel;D. Hess;S. Erlandsen;C. Wells
目的系统性念珠菌病是重症监护病房并发感染的主要原因。发病率和死亡率很高，即使在那些接受适当的抗真菌治疗。由于肠道被认为是系统性念珠菌病的主要入口，因此设计了实验来阐明酵母和丝状形式以及INT 1基因产物影响白色念珠菌粘附于肠上皮的能力。设计控制。单位：大学教学医院科研实验室。受试者成熟Caco-2和HT-29培养的肠细胞。干预措施以白色念珠菌INT 1突变株为研究对象，观察了白色念珠菌INT 1的超微结构表面相互作用。白色念珠菌与培养的肠上皮细胞，即Caco-2和HT-29细胞。这些突变株也被用来量化INT 1基因产物对C.白色念珠菌粘附（酵母和丝状形式）培养的肠上皮细胞。超微结构表面相互作用的C。用高分辨扫描电镜观察白念珠菌与培养肠上皮细胞的关系。C.通过使用比色酶联免疫吸附测定来定量白色念珠菌对培养的肠上皮细胞的粘附。测量和主要结果酵母和丝状C。白色念珠菌出现紧密粘附到培养的肠上皮细胞的顶面，和INT 1似乎有很少，如果有的话，这些超微结构的表面相互作用的影响。C.白色念珠菌丝似乎介导了对肠上皮细胞顶端微绒毛的粘附，趋触性（接触导向）似乎在C.白色念珠菌粘附。功能性INT 1的缺失与C.白色念珠菌酵母菌形成培养的肠上皮细胞。结论虽然功能性INT 1似乎促进了C.白色念珠菌酵母菌形式的培养肠上皮细胞，INT 1的作用，粘附的丝状形式是不清楚的，酵母和丝状形式可以坚持，并可能侵入，培养肠上皮细胞的顶面。
OBJECTIVE Systemic candidiasis is a major cause of complicating infections in intensive care units. Morbidity and mortality are high, even in those who receive appropriate antifungal therapy. Because the intestinal tract is considered a major portal of entry for systemic candidiasis, experiments were designed to clarify the ability of yeast and filamentous forms, as well as the INT1 gene product, to influence adherence of Candida albicans to the intestinal epithelium. DESIGN Controlled. SETTING University teaching hospital research laboratory. SUBJECTS Mature Caco-2 and HT-29 cultured enterocytes. INTERVENTIONS C. albicans INT1 mutant strains, defective in filament production, were used to observe the ultrastructural surface interactions of C. albicans with cultured intestinal epithelial cells, namely Caco-2 and HT-29 cells. These mutant strains also were used to quantify the effect of the INT1 gene product on C. albicans adherence (yeast and filamentous forms) to cultured enterocytes. Ultrastructural surface interactions of C. albicans with cultured enterocytes were observed with high resolution scanning electron microscopy. C. albicans adherence to cultured enterocytes was quantified by using a colorimetric enzyme-linked immunosorbent assay. MEASUREMENTS AND MAIN RESULTS Both yeast and filamentous forms of C. albicans appeared tightly adherent to the apical surface of cultured enterocytes, and INT1 appeared to have little, if any, effect on these ultrastructural surface interactions. The distal ends of C. albicans filaments appeared to mediate adherence to enterocyte apical microvilli, and thigmotropism (contact guidance) appeared to play a role in C. albicans adherence. The absence of functional INT1 was associated with decreased adherence of C. albicans yeast forms to cultured enterocytes. CONCLUSIONS Although functional INT1 appeared to facilitate adherence of C. albicans yeast forms to cultured enterocytes, the role of INT1 in adherence of filamentous forms was unclear, and both yeast and filamentous forms could adhere to, and perhaps invade, the apical surface of cultured enterocytes.