INCREASED EXPRESSION OF ENDOTHELIN-1 GENE IN BLOOD-VESSELS OF DEOXYCORTICOSTERONE ACETATE SALT HYPERTENSIVE RATS
INCREASED EXPRESSION OF ENDOTHELIN-1 GENE IN BLOOD-VESSELS OF DEOXYCORTICOSTERONE ACETATE SALT HYPERTENSIVE RATS
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DOI：
10.1161/01.hyp.21.6.916
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发表时间：
1993-06-01
期刊：
影响因子：
8.3
通讯作者：
SCHIFFRIN, EL
中科院分区：
文献类型：
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作者：
LARIVIERE, R;DAY, R;SCHIFFRIN, EL
我们最近发现，与单肾切除对照组大鼠相比，脱氧皮质酮-醋酸盐高血压大鼠血管酸性提取物中免疫反应性内皮素-1的含量增加。我们还通过免疫组织化学方法发现，DOCA-盐型高血压大鼠主动脉和肠系膜动脉内皮细胞内ET-1免疫反应明显增强。在本研究中，我们研究了DOCA-盐性高血压大鼠和单肾切除对照组大鼠血管中前内皮素-1基因的表达。用P-32标记的大鼠前内皮素-I互补RNA探针对319个碱基对的Northern印迹分析显示，DOCA-盐型高血压大鼠主动脉和肠系膜动脉中前内皮素-1信使RNA转录本的丰度增加了四到五倍。因此，DOCA-盐性高血压大鼠血管中免疫反应性内皮素-I含量的增加是继发于前内皮素-I基因表达的增加。在盐皮质激素性高血压中，前内皮素-1基因的过度表达可能参与了高血压的维持。
We have recently shown that the content of immunoreactive endothelin-1 is increased in acid extracts from blood vessels of deoxycorticosterone acetate (DOCA)-salt hypertensive rats compared with uninephrectomized control rats. We have also found by immunohistochemistry a significant increase in immunoreactive endothelin-1 in endothelial cells of aorta and mesenteric arteries of DOCA-salt hypertensive rats. In the present study, we investigated preproendothelin-1 gene expression in blood vessels of DOCA-salt hypertensive rats and uninephrectomized control rats. Northern blot analysis using a specific P-32-labeled complementary RNA probe for rat preproendothelin-I of 319 base pairs revealed a fourfold to fivefold increase in abundance of preproendothelin-1 messenger RNA transcripts in both aorta and mesenteric arteries from DOCA-salt hypertensive rats. Thus, increased immunoreactive endothelin-I content in blood vessels of DOCA-salt hypertensive rats is secondary to increased preproendothelin-I gene expression. Exaggerated expression of the preproendothelin-1 gene in mineralocorticoid hypertension may contribute to the maintenance of elevated blood pressure.