Composition of transcription factor B-TFIID.
Composition of transcription factor B-TFIID.
复制标题
转录因子B-TFIID的组成。
DOI：
10.1073/pnas.89.17.8140
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发表时间：
1992
影响因子：
11.1
通讯作者：
Sharp,PA
中科院分区：
文献类型：
--
作者：
Timmers,HT;Meyers,RE;Sharp,PA
RNA聚合酶II启动转录需要TFIID因子，该因子可以识别许多启动子共有的TATA元件。可以从哺乳动物细胞提取物中分离出两种不同的多亚基 TFIID 因子，它们都含有充分表征的 TATA 结合蛋白 (TBP)，并且能够在体外反应系统中支持 RNA 聚合酶 II 转录。纯化了较小的复合物 B-TFIID，并测定了其亚基组成。 B-TFIID 由两个亚基组成：TBP 和 170 kDa 的 TBP 相关因子 (TAF)。该 TAF 是 B-TFIID 特异性的，并且似乎不存在于 D-TFIID 复合物中。此外，还发现高度纯化的 B-TFIID 级分具有 (d)ATP 酶活性。
Initiation of transcription by RNA polymerase II requires a TFIID factor, which can recognize the TATA element common to many promoters. Two distinct multisubunit TFIID factors can be resolved from extracts of mammalian cells, and both of them contain the well-characterized TATA-binding protein (TBP) and are capable of supporting RNA polymerase II transcription in an in vitro reaction system. The smaller complex, B-TFIID, was purified and its subunit composition was determined. B-TFIID consists of two subunits: the TBP and a TBP-associated factor (TAF) of 170 kDa. This TAF is specific for B-TFIID and appears not to be present in the D-TFIID complex. Furthermore, it was found that the highly purified B-TFIID fractions have (d)ATPase activity.