Lactobacillus rhamnosus R11 consumed in a food supplement survived human digestive transit without modifying microbiota equilibrium as assessed by real-time polymerase chain reaction
Lactobacillus rhamnosus R11 consumed in a food supplement survived human digestive transit without modifying microbiota equilibrium as assessed by real-time polymerase chain reaction
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DOI：
10.1159/000106087
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发表时间：
2008-01-01
影响因子：
1.2
通讯作者：
Furet, Jean-Pierre
中科院分区：
文献类型：
--
作者：
Firmesse, Olivier;Mogenet, Agnes;Furet, Jean-Pierre
本研究旨在探讨鼠李糖乳杆菌R11和嗜酸乳杆菌R52在人消化道中的存活情况及其对消化道微生物群稳态的影响。我们设计了一个开放的人体试验，包括14名健康志愿者。在3周的发酵产品排除期之后，每天食用4粒含有2 × 10(9)鼠李糖乳杆菌R11和1 × 10(8)嗜酸乳杆菌R52的胶囊12天，并进行12天的冲洗期。采用实时聚合酶链反应对菌群的2株菌株和优势菌群进行定量。在胶囊消耗期结束时，在所有志愿者的粪便样本中检测到高水平的鼠李糖乳杆菌R11，平均达到7.1 log（10）菌落形成单位（CFU）当量/g粪便。仅1名志愿者的粪便中检测到嗜酸乳杆菌R52，最高水平为6.1 log(10) CFU当量/g粪便。平行进行稀释镀计数，结果不太一致，通常水平较低。未观察到食用胶囊对优势粪便种群的微生物群稳态有显著影响。球芽梭菌组、双歧杆菌组、拟杆菌组和瘦梭菌组粪便平均浓度分别为8.8、9.2、9.9和10.6 log(10) CFU当量/g。版权所有(c) 2008 S. Karger AG，巴塞尔。
The aim of this study was to evaluate the survival of Lactobacillus rhamnosus R11 and Lactobacillus acidophilus R52 in the human digestive tract and their effects on the microbiota homeostasis. We designed an open human trial including 14 healthy volunteers. A 3-week exclusion period of fermented products was followed by a 12-day consumption period of 4 capsules daily containing 2 x10(9) L. rhamnosus R11 and 1 x 10(8) L. acidophilus R52, and a 12-day wash-out period. The 2 strains and dominant bacterial groups of the microbiota were quantified by real-time polymerase chain reaction. At the end of the capsule consumption period, high levels of L. rhamnosus R11 were detected in faecal samples from all volunteers, reaching a mean value of 7.1 log(10) colony-forming unit (CFU) equivalents/g of stool. L. acidophilus R52 was detected in the stools of only 1 volunteer, reaching a maximum level of 6.1 log(10) CFU equivalents/g of stool. Dilution plating enumerations performed in parallel provided less consistent and generally lower levels. No significant effect of capsule consumption was observed on microbiota homeostasis for the dominant faecal populations. Mean values of 8.8, 9.2, 9.9 and 10.6 log(10) CFU equivalents/g of stool were obtained for the Clostridium coccoides, Bifidobacterium sp., Bacteroides sp. and Clostridium leptum groups, respectively. Copyright (c) 2008 S. Karger AG, Basel.