The PKCδ-Nrf2-ARE signalling pathway may be involved in oxidative stress in arsenic-induced liver damage in rats
The PKCδ-Nrf2-ARE signalling pathway may be involved in oxidative stress in arsenic-induced liver damage in rats
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DOI：
10.1016/j.etap.2018.05.012
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发表时间：
2018-09-01
影响因子：
4.3
通讯作者：
Zhang, Aihua
中科院分区：
文献类型：
--
作者：
Hu, Yong;Yu, Chun;Zhang, Aihua
砷中毒是一种影响成千上万人的世界性地方病。越来越多的动物、细胞和人体研究证据表明，砷对肝脏有害。氧化应激被认为是砷毒性肝损伤的主要机制。然而，其机制仍不清楚。鉴于这一事实，本研究的主要目的是研究蛋白激酶C δ-核因子E2相关因子2-抗氧化反应元件（PKC δ-Nrf 2-ARE）信号通路对肝损伤中氧化应激的影响。本研究采用本课题组建立的燃煤砷致大鼠肝损伤模型。检测氧化应激指标与PKC δ、Nrf 2、Keap 1、SOD 1、GPx 1的转录和蛋白表达水平，并进行相关性分析。结果表明，燃煤砷可引起大鼠氧化应激性肝损伤，其机制可能与PKC delta-Nrf 2-ARE信号通路有关。本研究为砷中毒机制的研究提供了新的途径。
Arsenic poisoning is a worldwide endemic disease that affects thousands of people. Growing evidence from animal, cell, and human studies indicates that arsenic has deleterious effects on the liver. Oxidative stress is considered the primary mechanism for arsenic toxicity in liver damage. However, the mechanisms remain unclear. In light of this fact, the main objective of this study was to investigate the effects of the protein kinase C delta-nuclear factor E2-related factor 2-antioxidant response element (PKC delta-Nrf2-ARE) signalling pathway on oxidative stress in liver damage. In the present study, we used a model of liver damage induced by coal-burning arsenic in rats, which was set up by our research group. The oxidative stress index and the transcription and protein expression levels of PKC delta, Nrf2, Keap1, SOD1, and GPx1 were detected, and then their correlation analyses were carried out. The results demonstrated that coal-burning arsenic can cause oxidative stress liver damage in rats, which may be related to the PKC delta-Nrf2-ARE signalling pathway. This study may provide a new pathway for studies of the mechanisms of arsenism.