The growth and development conditions in mouse offspring derived from ovarian tissue cryopreservation and orthotopic transplantation
The growth and development conditions in mouse offspring derived from ovarian tissue cryopreservation and orthotopic transplantation
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发表时间：
2020
期刊：
影响因子：
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通讯作者：
Jiang Zhu
中科院分区：
文献类型：
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作者：
Zhe Yan;Jiang Zhu
Abstract.Purpose To investigate the potential development or metabolic risk in offspring derived from mice with transplanted frozenthawed ovarian tissue..Methods Mice ovaries were intervened by vitrification (group V) and slow-freezing (group S) cryopreservation and orthotopic.transplantation. Orthotopic transplantation of fresh ovarian (group F) and natural mating (group C) served as control groups. The.fertility restoration and health conditions of generations were assessed by offspring counts, anti-fatigue and motor ability, and.organ morphology. The methylation rate and expression level of imprinted genes (IGF2R, H19, SNRPN, and PLAGL1) were.used to predict the potential risk of development in transplanted generations..Results Both the percentage of normal morphological follicles in different developmental periods and the litter size of receipt.mice were comparable in all three transplanted groups. There was no significant difference in offspring mice’s birth defects, body.weight gain, anti-fatigue ability, or exercise capacity among the four groups. The methylation rate of IGF2R, H19, and PLAGL1.showed a significant variation in cryopreservation groups as compared with control groups, as well as a difference in gene.expression. The SNRPN appeared to be stable in methylation status. There were no differences in mRNA expression in all.groups..Conclusions The different ovarian tissue cryopreservation methods did not influence either maternal fertility function or offspring.growth. However, these technologies could affect the methylation rate and expression level of some development-related.imprinting genes in the offspring, which may lead to some indeterminacy risk.
