Independence of measles-specific humoral and cellular immune responses to vaccination
Independence of measles-specific humoral and cellular immune responses to vaccination
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DOI：
10.1016/j.humimm.2012.02.016
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发表时间：
2012-05-01
期刊：
影响因子：
2.7
通讯作者：
Poland, Gregory A.
中科院分区：
文献类型：
--
作者：
Jacobson, Robert M.;Ovsyannikova, Irina G.;Poland, Gregory A.
通过一个更大、更独立的队列和更复杂的测量，我们试图证实我们的工作表明麻疹疫苗接种后体液和细胞免疫的独立性。我们从所有社会经济阶层招募了764名年龄分层的随机队列健康受试者，所有受试者均有2剂与年龄相适应的含麻疹疫苗的医疗记录。我们量化了麻疹特异性中和抗体水平，并检测了干扰素- γ (ifn - γ)酶联免疫吸附斑点法（ELISPOT）对麻疹病毒的反应。我们还通过酶联免疫吸附试验测量了外周血单核细胞（PBMCs）对麻疹病毒的反应中分泌的细胞因子，作为细胞免疫状态的次要指标。抗体水平的中位数和ifn - γ ELISPOT反应的中位数分别为844 mIU/mL（四分位数范围[IQR]: 418- 1752）和36 （IQR: 13.00-69.00）个斑点形成细胞（每2 × 10(5)个PBMCs）。我们只观察到非常弱的负相关（Spearman’s r(s)或p为-0.090[95%置信区间：-0.162-0.018]）。我们观察到在中和抗体水平和任何次要测量之间缺乏类似的定量重要相关性。我们的数据证实了第二剂麻疹疫苗接种后体液和细胞免疫反应的独立性。当研究人员追求新型麻疹疫苗和麻疹疫苗递送系统时，他们不能推断体液反应可以预测细胞反应。(C) 2012年美国组织相容性和免疫遗传学学会。Elsevier Inc.出版。版权所有。
With a larger, independent cohort and more sophisticated measures, we sought to confirm our work that indicated independence of humoral and cellular immunity following measles vaccination. We recruited an age-stratified random cohort of 764 healthy subjects from all socioeconomic strata, all with medical-record documentation of 2 age-appropriate doses of measles-containing vaccine. We quantified measles-specific neutralizing antibody levels and assayed the interferon-gamma (IFN-gamma) enzyme-linked immunosorbent spot assay (ELISPOT) response to measles virus. We also measured secreted cytokines from the peripheral blood mononuclear cells (PBMCs) in response to measles virus by performing enzyme-linked immunosorbent assays as secondary measures of cellular immune status. The median antibody level and median IFN-gamma ELISPOT response were 844 mIU/mL (interquartile range [IQR]: 418-1,752) and 36 (IQR: 13.00-69.00) spot-forming cells (per 2 x 10(5) PBMCs), respectively. We observed only a very weak and negative correlation (Spearman's r(s) or p of -0.090 [95% confidence interval: -0.162-0.018]). We observed a similar lack of quantitatively important correlations between the neutralizing antibody level and any of the secondary measures. Our data confirm the independence of humoral and cellular immune responses after the second dose of measles vaccination. As researchers pursue novel measles vaccine and measles vaccine delivery systems, they must not infer that humoral responses predict cellular responses. (C) 2012 American Society for Histocompatibility and Immunogenetics. Published by Elsevier Inc. All rights reserved.