Mammalian RNAi for the masses
Mammalian RNAi for the masses
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DOI：
10.1016/s0168-9525(02)00005-7
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发表时间：
2003-01-01
影响因子：
11.4
通讯作者：
Shi, Y
中科院分区：
文献类型：
--
作者：
Shi, Y
就在几年前，只有研究植物或秀丽隐杆线虫的生物学家才能使用RNA介导的干扰（RNAi）技术来深入了解基因功能。然而，最近的突破性发现，即体外合成的21至23个核苷酸的双链RNA可以作为小干扰RNA（siRNA）在哺乳动物细胞中引起基因特异性抑制，这使得RNAi在哺乳动物系统中也成为可能。仅在一年前报道，哺乳动物RNAi已经改变了我们研究高等真核生物基因功能的方式。而且，最近令人兴奋的进展允许通过DNA载体将siRNA递送到哺乳动物细胞中。除了提供化学合成siRNA的低成本替代方案外，这种基于DNA载体的策略能够介导稳定的靶基因抑制，从而允许在延长的时间段内进行基因功能分析。
Just a couple of years ago, only biologists working with plants or Caenorhabditis elegans could use RNA-mediated interference (RNAi) technology to gain insight into gene function. However, the recent groundbreaking discovery that in vitro synthesized, 21- to 23-nucleotide, double-stranded RNAs can act as small interfering RNAs (siRNAs) to elicit gene-specific inhibition in mammalian cells has made RNAi possible in mammalian systems too. Reported only a year ago, mammalian RNAi is already changing our way of studying gene function in higher eukaryotes. And, a recent exciting advance allows delivery of siRNAs into mammalian cells by a DNA vector. In addition to providing a low-cost alternative to the chemically synthesized siRNAs, this DNA-vector-based strategy is capable of mediating stable target gene inhibition, thus allowing gene function analysis over an extended period of time.