Enhanced Biodegradation of Methyl tert-butyl-ether by a Microbial Consortium
Enhanced Biodegradation of Methyl tert-butyl-ether by a Microbial Consortium
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微生物群落增强甲基叔丁基醚的生物降解
DOI：
10.1007/s00284-013-0480-9
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发表时间：
2014-03-01
影响因子：
2.6
通讯作者：
Yan, Wei
中科院分区：
文献类型：
--
作者：
Li, Shan-shan;Zhang, Di;Yan, Wei
甲基叔丁基醚（MTBE）作为汽油添加剂的广泛使用，导致地下水系统中MTBE的检出率较高。因此，研究人员对mtbe影响下的含水层的生物修复越来越关注。本文对一个mtbe直接降解菌群（命名为RS1）进行了富集和进一步研究。为了鉴定该财团的微生物群落，分别从营养培养基和MSM培养基（以MTBE为唯一碳源）中分离出17株和12株不同的单株。基于16S rdna的系统发育分析显示，这些细菌分属14属，其中假单胞菌占优势地位。此外，还鉴定出了几种以MTBE为唯一碳源和能量源生长的菌株，如M1，与降解MTBE的节杆菌sp. ATCC27778有关。此外，在财团RS1 （M1:RS1 = 1:2）中适当添加一定的单一菌株，通过增加MTBE高效降解菌的数量，促进MTBE的降解。该研究将为mtbe污染含水层的进一步生物修复提供微生物来源和理论基础，具有应用潜力和环境意义。
The widespread use of Methyl tert-butyl-ether (MTBE) as a gasoline additive has resulted in a higher detection rate of MTBE in groundwater systems. Therefore, the researchers show more concern about the bioremediation of MTBE-impacted aquifers. In this paper, a MTBE-direct-degrading bacterial consortium was enriched (named RS1) and further studied. In order to identify the microbial community of the consortium, 17 and 12 different single strains were isolated from nutrient medium and MSM media (with MTBE as the sole carbon source), respectively. 16S rDNA-based phylogenetic analysis revealed that these diverse bacteria belonged to 14 genera, in which Pseudomonas was dominant. Several strains which can grow with MTBE as the sole carbon and energy source were also identified, such as M1, related to MTBE-degrading Arthrobacter sp. ATCC27778. Furthermore, the appropriate addition of certain single strain in consortium RS1 (M1:RS1 = 1:2) facilitates MTBE degradation by increasing the quantity of efficient MTBE-degrading bacteria. This work will provide microbial source and theoretical fundament for further bioremediation of MTBE-contaminated aquifers, which has applied potential and environmental importance.