Efficient functional screening of a cellular cDNA library to identify severe fever with thrombocytopenia syndrome virus entry factors
Efficient functional screening of a cellular cDNA library to identify severe fever with thrombocytopenia syndrome virus entry factors
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DOI：
10.1038/s41598-020-62876-1
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发表时间：
2020-04-07
影响因子：
4.6
通讯作者：
Saijo，Masayuki
中科院分区：
文献类型：
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作者：
Shimojima，Masayuki;Sugimoto，Satoko;Saijo，Masayuki
识别病毒进入的宿主细胞因子对病毒趋向性的分子解释是有用的，通常会导致对病毒诱导疾病的更深刻的理解。发热伴血小板减少综合征（SFTS）是一种由SFTS病毒引起的新兴传染病。没有对付这种疾病的对策。在本报告中，我们展示了一种利用病毒样颗粒对细胞cDNA文库进行功能筛选的有效方法，以鉴定SFTS病毒的进入因子。从人肝脏cDNA文库中成功鉴定出两个编码树突状细胞特异性ICAM-3捕获非整合素相关（DC-SIGNR）的变体，这是一种钙依赖性凝集素，已知可增强SFTS病毒感染。我们将讨论尚不确定的SFTS病毒进入因子和与SFTS病毒有关的其他病毒进入因子的申请。
The identification of host cell factors for virus entry is useful for the molecular explanation of viral tropisms and often leads to a more profound understanding of virus-induced diseases. Severe fever with thrombocytopenia syndrome (SFTS) is an emerging infectious disease caused by SFTS virus. No countermeasures against the disease exist. In this report, we show an efficient method using virus-like particles for the functional screening of a cellular cDNA library to identify SFTS virus entry factors. Two variants encoding dendritic cell-specific ICAM-3 grabbing non-integrin related (DC-SIGNR), a calcium-dependent lectin known to enhance SFTS virus infection, were successfully identified from a human liver cDNA library. We will discuss applications for yet unidentified factor(s) for SFTS virus entry and for entry factor(s) for other viruses related to SFTS virus.