Inducing substances for chondrogenic differentiation of dental pulp stem cells in the conditioned medium of a novel chordoma cell line
Inducing substances for chondrogenic differentiation of dental pulp stem cells in the conditioned medium of a novel chordoma cell line
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DOI：
10.1007/s13577-021-00662-5
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发表时间：
2022-01
期刊：
影响因子：
4.3
通讯作者：
Hiroyoshi Kino;H. Akutsu;H. Ishikawa;S. Takano;S. Takaoka;Junko Toyomura;Takuma Hara;E. Ishikawa;Y. Matsumaru;H. Bukawa;A. Matsumura
中科院分区：
文献类型：
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作者：
Hiroyoshi Kino;H. Akutsu;H. Ishikawa;S. Takano;S. Takaoka;Junko Toyomura;Takuma Hara;E. Ishikawa;Y. Matsumaru;H. Bukawa;A. Matsumura
我们成功建立了源自斜坡脊索瘤的脊索瘤细胞系，命名为TSK-CHO1。目前，只有一种颅底脊索瘤细胞系 UM-chor1 可供研究人员免费使用。免疫细胞化学染色或条件培养基 (CM) ELISA 表明，建立的 TSK-CHO1 细胞是肿瘤性的，表现出多形性特征，并分泌短链细胞。细胞还分泌 SOX9，从而增强短尾草的产量。 TSK-CHO1细胞的CM在人牙髓干细胞（DPSC）分化为纤维软骨细胞的过程中促进透明质酸和II型胶原的产生。将 DPSC 颗粒在补充有 10% TSK-CHO1 细胞 CM 的生长培养基中培养 2 周，可在含氧量正常的条件下诱导纤维软骨组织。 TSK-CHO1 细胞产生的 Brachyury 以剂量依赖性方式促进软骨特有的 II 型胶原蛋白的产生。新建立的颅底脊索瘤细胞系TSK-CHO1有望用于阐明颅底脊索瘤的发病机制并研究纤维软骨产生的机制。
We successfully established a chordoma cell line, designated TSK-CHO1, derived from the clival chordoma. Currently, there is only one skull base chordoma cell line, UM-chor1, freely available to researchers. The established TSK-CHO1 cells were neoplastic, exhibited pleomorphic features, and secreted brachyury, as revealed by immunocytochemical staining or ELISA of conditioned medium (CM). Cells also secreted SOX9, which enhanced brachyury production. The CM of TSK-CHO1 cells promoted the production of hyaluronic acid and type II collagen during differentiation of human dental pulp stem cells (DPSCs) into fibrocartilage cells. Culture of DPSC pellets in a growth medium supplemented with 10% CM of TSK-CHO1 cells for 2 weeks resulted in the induction of fibrocartilage tissue under normoxic conditions. Brachyury produced by TSK-CHO1 cells promoted the production of collagen type II, peculiar to cartilage, in a dose-dependent manner. The newly established skull base chordoma cell line, TSK-CHO1, is expected to be used for elucidating the pathogenesis of skull base chordoma and for investigating the mechanism underlying the production of fibrocartilage.