Insulin-like growth factor-I stimulates oxytocin and progesterone production by bovine granulosa cells in culture.
Insulin-like growth factor-I stimulates oxytocin and progesterone production by bovine granulosa cells in culture.
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胰岛素样生长因子-I 刺激培养的牛颗粒细胞产生催产素和黄体酮。
作者：
D. Schams;R. Koll;C. Li
研究了胰岛素样生长因子-I（IGF-I）、表皮生长因子（EGF）、成纤维细胞生长因子（FGF）和神经生长因子（NGF）对体外培养的牛颗粒细胞和黄体细胞分泌催产素和孕酮的影响。催产素的分泌刺激，以剂量依赖性的方式，IGF-I在48和120小时的培养水平远远高于LH，FSH，EGF，FGF或NGF刺激后。IGF-I对孕酮也有类似的作用，但与催产素相反，EGF、NGF或FGF并不增加孕酮的分泌。在原代培养过程中，4小时，分散的牛黄体细胞获得的黄体之间的第4和第10天的发情周期，所有的生长因子测试未能刺激催产素或孕酮的分泌。这些数据表明生长因子（尤其是IGF-I）与卵巢生理学的相关性及其对卵泡分化和黄体化的可能重要性。
The effect of insulin-like growth factor-I (IGF-I), epidermal growth factor (EGF), fibroblast growth factor (FGF) and nerve growth factor (NGF) on production of oxytocin and progesterone by cultured bovine granulosa and luteal cells was studied. Secretion of oxytocin was stimulated, in a dose-dependent manner, by IGF-I at 48 and 120 h of culture to levels much higher than those after stimulation with LH, FSH, EGF, FGF or NGF. A similar effect of IGF-I was observed for progesterone but, in contrast to oxytocin, secretion of progesterone was not increased by EGF, NGF or FGF. During primary culture, for 4 h, of dispersed bovine luteal cells obtained from corpora lutea between days 4 and 10 of the oestrous cycle, all the growth factors tested failed to stimulate secretion of oxytocin or progesterone. The data suggest the relevance of growth factors (especially IGF-I) for ovarian physiology and their possible importance for differentiation of follicles and luteinization.